Compositions and methods for the biosynthesis of vanillan or vanillin beta-D-glucoside
Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside.
1. A recombinant mutant Arom Multifunctional Enzyme (AROM) polypeptide having the amino acid sequence of SEQ ID NO:20 or SEQ ID NO:22.
2. The recombinant mutant AROM polypeptide of claim 1 , wherein said recombinant mutant AROM polypeptide further comprises a purification tag, a chloroplast transit peptide, a mitochondrial transit peptide, an amyloplast peptide, signal peptide, or a secretion tag at the N- or C-terminus of said polypeptide.