Method of diagnosing surgical site infections
View Patent ↗A marker useful in diagnosing surgical site infections is provided. In the method of the present invention for detecting surgical site infections, sCD14-ST in a sample is measured.
1. A method of detecting and treating surgical site infections not accompanied by systemic inflammatory response syndrome, comprising the steps of:
(A) collecting a sample from a patient after surgery suspected of having a surgical site infection not accompanied by systemic inflammatory response syndrome or a patient suffering from a surgical site infection not accompanied by systemic inflammatory response syndrome;
(B) measuring sCD14-ST in the sample;
(C) comparing the amount of sCD14-ST in the sample to a threshold value;
(D) diagnosing that the patient suffers from a surgical site infection not accompanied by systemic inflammatory response syndrome when an sCD14-ST value is higher than that of an uninfected person; and
(E) administering a treatment to the patient for the surgical site infection using an antimicrobial treatment.
2. The method according to claim 1 , wherein the sCD14-ST value is compared to the threshold previously determined selected from the group consisting of a healthy person group, a non-infection group, an infection group, a sepsis group, septic groups of different pathological level, and septic groups of different pathological types.
3. The method according to claim 1 , wherein sCD14-ST is measured by an immunoassay.
4. The method of claim 1 , wherein sCD14-ST is measured in the sample within about 7 to 10 days after surgery.
5. A method of detecting and treating surgical site infections not accompanied by systemic inflammatory response syndrome, comprising the steps of:
(A) collecting a sample from a patient after surgery suspected of having a surgical site infection not accompanied by systemic inflammatory response syndrome or a patient suffering from a surgical site infection not accompanied by systemic inflammatory response syndrome;
(B) measuring sCD14-ST in the sample by an immunoassay using a kit for detecting surgical site infections not accompanied by systemic inflammatory response syndrome, comprising:
(i) an antibody specific to sCD14-ST, said antibody being immobilized onto a latex carrier, magnetic particles or a substrate, or being labeled with a labeling substance,
(ii) standard data showing a correlation between the amount of sCD14-ST in a sample and surgical site infections not accompanied by systemic inflammatory response syndrome,
(C) comparing the amount of sCD14-ST in the sample to a threshold value;
(D) diagnosing that the patient suffers from a surgical site infection not accompanied by systemic inflammatory response syndrome when an sCD14-ST value is higher than that of an uninfected person; and
(E) administering a treatment to the patient for the surgical site infection using an antimicrobial treatment.
6. The method of claim 5 , wherein sCD14-ST is measured in the sample within about 7 to 10 days after surgery.
7. The method of claim 1 , wherein sCD14-ST is measured in the sample within about 5 to 10 days after surgery.
8. The method of claim 5 , wherein sCD14-ST is measured in the sample within about 5 to 10 days after surgery.
9. The method of claim 1 , wherein sCD14-ST is measured using a kit for detecting surgical site infections not accompanied by systemic inflammatory response syndrome, the kit comprising:
(a) an antibody specific to sCD14-ST, said antibody being immobilized onto a latex carrier, magnetic particles or a substrate, or being labeled with a labeling substance,
(b) standard data showing a correlation between the amount of sCD14-ST in a sample and surgical site infections not accompanied by systemic inflammatory response syndrome, and
(c) an instruction manual.