System and method for detecting components of a mixture including tooth elements for alignment
View Patent ↗Examples are described including assay platforms having tooth elements. An impinging element may sequentially engage tooth elements on the assay platform to sequentially align corresponding detection regions with a detection unit. In this manner, multiple measurements may be made of detection regions on the assay platform without necessarily requiring the starting and stopping of a motor.
1. An assay system comprising:
an assay platform comprising multiple detection regions and corresponding tooth elements;
a motor coupled to the assay platform, wherein the motor is configured to move the assay platform such that the detection regions move along a first path and the tooth elements move along a second path;
an impinging element configured for placement in a first position that allows for movement of the assay platform and a second position, wherein the impinging element engages at least one tooth element when in the second position;
a detection unit configured to detect an analyte, wherein the detection unit is positioned such that when the impinging element is in the second position, the detection unit is aligned with at least one of the multiple detection regions; and
a controller communicatively coupled to the impinging element and the motor, wherein the controller is configured to provide a control signal to the impinging element to place the impinging element in the first position or the second position.
2. The assay system of claim 1 , wherein the assay platform comprises a circular substrate defining multiple tooth elements along a circumference of the circular substrate.
3. The assay system of claim 1 , wherein at least one of the multiple detection regions corresponds to an extended tooth element having a different dimension than others of the tooth elements.
4. The assay system of claim 3 , wherein the impinging element is further configured for placement in a third position, in which the impinging element engages the extended tooth element.
5. The assay system of claim 1 , wherein the controller is configured to move the impinging element to sequentially engage with each tooth element of the tooth elements.
6. The assay system of claim 5 , wherein the controller is further configured to stop the motor for a predetermined interval after the impinging element engages with each tooth element.
7. The assay system of claim 1 , wherein the detection region is configured to contain any of a biological and a clinical sample.
8. The assay system of claim 1 , wherein the detection unit includes an optical light sensor.
9. The assay system of claim 1 , wherein the detection regions configured to move along a first path are a first set of detection regions, and wherein the assay platform further comprises a second set of detection regions, the second set of detection regions configured to move along a third path, wherein the detection unit is configured to shift from a position associated with the first path to a position associated with the second path.
10. The assay system of claim 1 , wherein the assay platform comprises a substrate formed from an injection molded polymer.
11. The assay system of claim 1 , wherein the controller is configured to stop the motor while the detection unit performs a measurement of at least one detection region.
12. The assay system of claim 1 , wherein the motor is a brushed DC motor.
13. The assay system of claim 1 , wherein the first and second paths are circular.
14. The assay system of claim 13 , wherein the motor is configured to move the assay platform to effect a separation of one or more mixtures placed in the detection regions responsive to a centrifugal force.
15. The assay system of claim 14 , wherein the mixture is a fluid sample, wherein the fluid sample includes a plurality of beads having complexes formed thereon, individual ones of the complexes comprising a target analyte and a labeling agent, wherein the fluid sample further includes a free labeling agent, and wherein the beads in the fluid sample are transported responsive to the centrifugal force.
16. A method for performing an assay, the method comprising:
moving, by a motor, multiple detection regions of an assay platform along a first path and corresponding tooth elements along a second path;
aligning a detection unit with a detection region on the assay platform at least in part by moving, using a controller, an impinging element from a first position and a second position, wherein the impinging element is in the second path when in the second position; and
performing a measurement on the detection region using the detection unit.
17. The method of claim 16 further comprising serially engaging the impinging element with multiple ones of the tooth elements for a predetermined interval at each tooth element.
18. The method of claim 17 , wherein the measurement is performed during the predetermined interval.
19. The method of claim 16 , wherein the assay platform comprises a circular substrate defining multiple tooth elements along a circumference of the circular substrate.
20. The method of claim 19 , wherein the first path is circular.
21. The method of claim 20 wherein moving the detection regions applies a centrifugal force to one or more mixtures in the detection regions.
22. The method of claim 21 , wherein the centrifugal force effects a separation of one or more components of the mixtures.
23. The assay system of claim 22 , wherein the mixture is a fluid sample, wherein the fluid sample includes a plurality of beads having complexes formed thereon, individual ones of the complexes comprising a target analyte and a labeling agent, wherein the fluid sample further includes a free labeling agent, and wherein the beads in the fluid sample are transported responsive to the centrifugal force.
24. The method of claim 16 further comprising moving a second set of detection regions of the assay platform along a third path, wherein the detection unit is configured to shift from a position associated with the first path to a position associated with the second path, and wherein the detection regions configured to move along a first path are a first set of detection regions.
25. An assay platform comprising:
a substrate at least partially defining multiple detection regions, the multiple detection regions positioned at a same radium from a center of the substrate;
a first tooth element extending a first distance away from a center of the substrate, wherein the first tooth element is associated with one of the plurality of detection regions; and
multiple second tooth elements, each multiple second tooth element associated with a remaining one of the multiple detection regions, wherein the multiple second tooth elements extend a second distance away from the center of the substrate, and wherein the first distance is larger than the second distance.
26. The assay platform of claim 25 , wherein the multiple detection regions are configured to move along a first path.
27. The assay platform of claim 26 , wherein the first path is circular.
28. The assay platform of claim 27 wherein moving the detection regions applies a centrifugal force to one or more mixtures in the detection regions.
29. The assay platform of claim 28 , wherein the centrifugal force effects a separation of one or more components of the mixtures.
30. The assay platform of claim 29 , wherein the mixture is a fluid sample, wherein the fluid sample includes a plurality of beads having complexes formed thereon, individual ones of the complexes comprising a target analyte and a labeling agent, wherein the fluid sample further includes a free labeling agent, and wherein the beads in the fluid sample are transported responsive to the centrifugal force.