Nucleic acid sequences encoding transcription factors regulating alkaloid biosynthesis and their use in modifying plant metabolism
View Patent ↗Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.
1. An isolated cDNA molecule comprising a nucleotide sequence selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2;
(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3; and
(c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b), and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
2. An expression vector comprising the cDNA molecule of claim 1 , operably linked to one or more control sequences suitable for directing expression in a Nicotiana host cell.
3. A genetically engineered nicotinic alkaloid-producing Nicotiana plant comprising a cell comprising a chimeric nucleic acid construct comprising the isolated cDNA molecule of claim 1 , wherein the isolated cDNA molecule is linked to a heterologous nucleic acid to form the chimeric nucleic acid construct.
4. The engineered Nicotiana plant of claim 3 , wherein the plant is a Nicotiana tabacum plant.
5. Seeds from the engineered Nicotiana plant of claim 3 , wherein the seeds comprise the chimeric nucleic acid construct.
6. A product comprising the engineered Nicotiana plant of claim 3 .
7. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is set forth in SEQ ID NO: 1.
8. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is set forth in SEQ ID NO: 2.
9. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3.
10. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 90% identical to the nucleotide sequence of SEQ ID NO: 1, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
11. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 90% identical to the nucleotide sequence of SEQ ID NO: 2, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
12. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 90% identical to the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
13. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 95% identical to the nucleotide sequence of SEQ ID NO: 1, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
14. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 95% identical to the nucleotide sequence of SEQ ID NO: 2, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
15. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 95% identical to the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
16. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 96% identical to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
17. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 96% identical to the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
18. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 97% identical to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
19. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 97% identical to the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
20. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 98% identical to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
21. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 98% identical to the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
22. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 99% identical to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.
23. The isolated cDNA molecule of claim 1 , wherein the nucleotide sequence is at least 99% identical to the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 3, and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis.