METHOD OF MAKING A FORMULATION FOR DEACTIVATING NUCLEIC ACIDS
The disclosure relates to formulations for use in deactivating nucleic acids and methods of making and using the same.
1 . A method of making a formulation for deactivating nucleic acids, the method comprising the steps of:
(a) separately dissolving solid forms of a corrosion-inhibiting agent and a wetting agent, the corrosion-inhibiting agent being phosphate, borate or sodium bicarbonate, and the wetting agent being a surfactant or detergent;
(b) combining together the dissolved forms of the corrosion-inhibiting agent and the wetting agent to form a first mixture;
(c) combining together the first mixture with a solubilizing agent to form a second mixture, the solubilizing agent being an organic solvent or an emulsifying agent; and
(d) instructing a user to combine the second mixture with a deactivating agent to form the formulation, the deactivating agent being sodium hypochlorite or dichloroisocyanurate,
whereby the concentration of the corrosion-inhibiting agent in the formulation is from about 10 mM to about 750 mM;
whereby the concentration of the wetting agent in the formulation is from about 0.005% to about 1% (w/v);
whereby the concentration of the solubilizing agent in the formulation is from about 0.001% to about 20% (v/v);
whereby the concentration of the deactivating agent in the formulation is from about 0.06% to about 3% (w/v) if the deactivating agent is sodium hypochlorite;
whereby the concentration of the deactivating agent in the formulation is from about 5 mM to about 400 mM if the deactivating agent is dichloroisocyanurate; and
whereby each agent of the formulation will remain substantially in solution at 22° C.
2 . The method of claim 1 , wherein the deactivating agent is sodium hypochlorite.
3 . The method of claim 2 , wherein the sodium hypochlorite is to be present in the formulation at a concentration of from about 0.18% to about 1.8% (w/v).
4 . The method of claim 2 , wherein the sodium hypochlorite is to be present in the formulation at a concentration of from about 0.6% to about 1.5% (w/v).
5 . The method of claim 2 , wherein the sodium hypochlorite is to be present in the formulation at a concentration of from about 0.6% to about 1.2% (w/v).
6 . The method of claim 1 , wherein the deactivating agent is dichloroisocyanurate.
7 . The method of claim 6 , wherein the dichloroisocyanurate is present in the formulation at a concentration of from about 10 mM to about 200 mM.
8 . The method of claim 6 , wherein the dichloroisocyanurate is present in the formulation at a concentration of from about 20 mM to about 100 mM.
9 . The method of claim 6 , wherein the dichloroisocyanurate is present in the formulation at a concentration of from about 40 mM to about 80 mM.
10 . The method of claim 1 , wherein the corrosion-inhibiting agent is sodium bicarbonate.
11 . The method of claim 1 , wherein the wetting agent is SDS or LLS.
12 . The method of claim 1 , wherein the solubilizing agent is an organic solvent.
13 . The method of claim 12 , wherein the organic solvent is benzyl acetate, polysorbate 20 (PS20), or isopropanol.
14 . The method of claim 1 , wherein the solubilizing agent is an emulsifying agent.
15 . The method of claim 14 , wherein the emulsifying agent is polyoxyethylene sortitan mono-palmitate, lecithin, or ethylene glycol distearate.
16 . The method of claim 1 , wherein the solubilizing agent is a fragrance.
17 . The method of claim 1 , further comprising dissolving a solid form of the solubilizing agent prior to step (c).
18 . The method of claim 1 , wherein the second mixture is 600 mM sodium bicarbonate, pH 9.3, 0.1% SDS, and 0.05% fragrance.
19 . The method of claim 1 , wherein the formulation is 0.6% sodium hypochlorite, 90 mM sodium bicarbonate, pH 9.3, 0.015 SDS, and 0.0075% fragrance.