Chelated nanoceria for the treatment of oxidative stress
View Patent ↗A process for making cerium-containing nanoparticles with biocompatible stabilizers is described, wherein an aqueous reaction mixture comprising cerous ion, citric acid, a stabilizer (chelator) selected from the group consisting of nitrilotriacetic acid, ethylene glycol tetraacetic acid and diethylenetriaminepentaacetic acid, and an oxidant, is provided, followed by a heating step to effectively form the nanoparticles. These biocompatible nanoparticles can be used to treat oxidative stress related diseases and events, such as ischemic stroke.
1. A method of making a dispersion of nanoparticles, comprising:
a. forming a reaction mixture comprising cerous ion, citric acid, a stabilizer, an oxidant, and water, wherein the stabilizer is selected from the group consisting of nitrilotriacetic acid, ethylene glycol tetraacetic acid and diethylenetriaminepentaacetic acid; and
b. forming a dispersion of cerium-containing nanoparticles in the reaction mixture.
2. The method of claim 1 , further comprising heating or cooling the reaction mixture.
3. The method of claim 1 , wherein the temperature of said reaction mixture is less than or equal to the boiling temperature of water.
4. The method of claim 1 , wherein said cerium-containing nanoparticles comprise an oxide of cerium.
5. The method of claim 1 , wherein said cerium-containing nanoparticles are crystalline.
6. The method of claim 5 , wherein said nanoparticles are characterized by a cubic fluorite crystal structure.
7. The method of claim 1 , wherein said oxidant comprises hydrogen peroxide.
8. The method of claim 1 , wherein said cerium-containing nanoparticles are substantially non-agglomerated.
9. The method of claim 1 , wherein said dispersion of cerium-containing nanoparticles is stable against particle agglomeration and settling for greater than 12 months.
10. The method of claim 1 , wherein said dispersion of cerium-containing nanoparticles is stable against particle agglomeration and settling for about 12 months.
11. The method of claim 1 , wherein said dispersion of cerium-containing nanoparticles has a zeta-potential ranging from about −15 mV to about −30 mV.
12. The method of claim 1 , wherein said cerium-containing nanoparticles are characterized by a hydrodynamic diameter less than 10 nanometers.
13. The method of claim 1 , wherein said cerium-containing nanoparticles are characterized by a hydrodynamic diameter of about 10 nanometers.