Single step desiccating bead-in-syringe concentrating device

1. A device for processing a biological material comprising:

a syringe barrel having an inner diameter symmetrical about a longitudinal axis, an open end, a closed end, and an outlet at the closed end;

desiccating beads positioned within the syringe barrel;

a filter positioned at the closed end of the syringe barrel; and

a plunger insertable into the syringe barrel through the open end, the plunger including:

an exterior potion extending from within the syringe barrel, and

a paddle assembly completely located within the syringe barrel during use, the paddle assembly configured to break into a plurality of pieces when the plunger is depressed and the paddle assembly is pressed against the filter, the paddle assembly fixed to the plunger and located between the plunger and the filter,

wherein the paddle assembly is configured to mix the biological material with the desiccating beads.

2. The device of claim 1 , wherein the beads comprise polystyrene, polyacrylamide, glass, or metal.

3. The device of claim 2 , wherein the beads are polyacrylamide beads.

4. The device of claim 1 , wherein the filter comprises pores with diameters smaller than the beads.

5. The device of claim 4 , wherein the pores are larger than a diameter of the biological material.

6. The device of claim 1 , wherein the paddle assembly comprises a stem that extends along the longitudinal axis of the syringe barrel and a plurality of paddles extending radially from the stem.

7. The device of claim 1 , wherein the device is configured to withdraw the biological material from a patient, process the biological material, and return the processed biological material back to the patient.

8. The device of claim 7 , wherein process the biological material comprises concentrating the biological material.

9. The device of claim 8 , wherein the biological material is whole blood, bone marrow, adipose tissue, or chondrocytes.

10. The device of claim 7 , wherein the biological material is whole blood comprising macrophages, and contact with the beads induces the macrophages to polarize into an M2 phenotype.

11. The device of claim 1 , wherein the filter is positioned within the syringe barrel at the closed end or outside the syringe barrel at the outlet.

12. A device for processing a biological material comprising:

a syringe barrel extending along a longitudinal axis from an open end to a closed end, and having an outlet at the closed end, wherein polyacrylamide beads are positioned within the syringe barrel;

a filter positioned at the closed end of the syringe barrel, wherein the filter allows biological materials to pass through, but not the polyacrylamide beads;

a plunger insertable into the syringe barrel through the open end, the plunger having a paddle assembly, wherein the paddle assembly comprises a stem that extends along the longitudinal axis of the syringe barrel and a plurality of paddles that extend radially from the stem, the stem configured to break into a plurality of pieces when the plunger is depressed and the paddle assembly is pressed against the filter; and

a needle,

wherein the device is configured to aspirate a biological material from a patient, process the biological material, and return the processed biological material back to the patient.

13. The device of claim 12 , wherein the biological material is bone marrow aspirate, the polyacrylamide beads concentrate the bone marrow aspirate to yield concentrated bone marrow aspirate, and concentrated bone marrow aspirate is expelled from the device when the plunger is depressed.

14. The device of claim 12 , wherein the biological material is whole blood, adipose tissue, or a combination thereof, and wherein an autologous protein solution is generated when the whole blood, adipose tissue, or combination thereof is contacted with the polyacrylamide beads.

15. The device of claim 12 , wherein the filter is positioned inside the syringe barrel at the closed end.

16. The device according to claim 15 , further comprising a second filter removably coupled outside the syringe barrel to the outlet.

17. The device of claim 12 , wherein the plurality of paddles are configured to break into a plurality of pieces when the plunger is depressed and the paddle assembly is pressed against the filter.

18. A device for processing a biological material comprising:

a syringe barrel having an inner diameter symmetrical about a longitudinal axis, an open end, a closed end, and an outlet at the closed end;

desiccating beads positioned within the syringe barrel, each of the desiccating beads having a diameter;

a filter positioned within the syringe barrel at the closed end, the filter defining pores having diameters that are smaller than the diameter of each of the desiccating beads; and

a plunger insertable into the syringe barrel through the open end, the plunger including a paddle assembly configured to collapse and break into a plurality of pieces when the plunger is depressed and the paddle assembly is pressed against the filter, the paddle assembly fixed to the plunger,

wherein the paddle assembly is configured to mix the biological material with the desiccating beads when the plunger and paddle assembly are rotated within the syringe barrel.

19. The device of claim 18 , wherein the beads comprise at least one of polystyrene, polyacrylamide, glass, or metal.

20. The device of claim 18 , wherein the diameters of the pores are larger than a diameter of the biological material.

21. The device of claim 18 , wherein the paddle assembly comprises a stem that extends along the longitudinal axis of the syringe barrel and a plurality of paddles extending radially from the stem.

22. The device of claim 18 , wherein the device is configured to withdraw the biological material from a patient, process the biological material, and return the processed biological material back to the patient.