Humanized anti-CCR2 antibodies and methods of use therefor
View Patent ↗The present invention relates to a humanized antibody or functional fragment thereof which binds to a mammalian (e.g., human) CC-chemokine receptor 2 (CCR2) or a portion of the receptor and blocks binding of a ligand to the receptor. The invention further relates to a method of inhibiting the interaction of a cell bearing mammalian CCR2 with a ligand thereof, and to use of the antibodies and fragments in therapeutic, prophylactic and diagnostic methods.
1. A humanized immunoglobulin or antigen binding portion thereof having binding specificity for CCR2 comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequences of SEQ ID NO:17 and the amino acid sequence of SEQ ID NO:110 or a portion thereof, and wherein the light chain comprises the amino acid sequence of SEQ ID NO:12 and the amino acid sequence of SEQ ID NO:112.
2. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the heavy chain has a variable region and a constant region, and the variable region is encoded by the nucleic acid sequence of SEQ ID NO:108.
3. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the heavy chain has a variable region and a constant region, and the constant region is encoded by the nucleic acid sequence of SEQ ID NO:111 or a portion thereof.
4. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the heavy chain has a variable region and a constant region, and the variable region is encoded by the nucleic acid sequence of SEQ ID NO:108 and the constant region is encoded by the nucleic acid sequence of SEQ ID NO:111 or a portion thereof.
5. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the light chain has a variable region and a constant region, and the variable region is encoded by the nucleic acid sequence of SEQ ID NO:109.
6. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the light chain has a variable region and a constant region, and the constant region is encoded by the nucleic acid sequence of SEQ ID NO:113.
7. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the light chain has a variable region and a constant region, and the variable region is encoded by the nucleic acid sequence of SEQ ID NO:109 and the constant region is encoded by the nucleic acid sequence of SEQ ID NO:113.
8. The humanized immunoglobulin or antigen binding portion thereof of claim 1 , wherein the heavy chain has a variable region and a constant region, and the heavy chain variable region is encoded by the nucleic acid sequence of SEQ ID NO:108 and the heavy chain constant region is encoded by the nucleic acid sequence of SEQ ID NO:111 or a portion thereof, and wherein the light chain has a variable region and a constant region, and the light chain variable region is encoded by the nucleic acid sequence of SEQ ID NO:109 and the light chain constant region is encoded by the nucleic acid sequence of SEQ ID NO:113.