Rapid detection of analytes in liquid samples
A device for detecting at least one analyte in a liquid sample generally comprises (i) a support having a chamber for receiving a biological fluid (e.g., milk) therein, wherein said chamber is an elongate chamber having a length axis; (ii) a (stationary or movable) carrier (in some embodiments in the form of an end cap, or connected to an end cap; in other embodiments in the form of an agitator in said elongate chamber).
1. An XYZ stage assembly for securing a sample cartridge in an automated microscope having X, Y, and Z planes of movement, the XYZ stage assembly comprising:
a sample cartridge having an end portion, a pair of generally parallel opposing side edge portions, and a locking edge portion formed thereon; and
an XYZ stage, said XYZ stage comprising:
a base member having a planar stage surface portion;
a pair of generally parallel oppositely facing guide members on said planar stage surface portion and configured for slideably receiving said sample cartridge therebetween;
a locking member on said planar stage surface portion and positioned to press against the sample cartridge locking edge portion when said sample cartridge is inserted between said pair of generally parallel oppositely facing guide members, so that pressure is exerted by said locking member through said sample cartridge against at least one of said pair of generally parallel oppositely facing guide members, whereby said sample cartridge is removably locked in place on the XYZ stage in the Z plane,
wherein said locking member comprises a ball detent;
wherein each pair of generally parallel opposing side edge portions has an upper corner portion, with said locking edge portion positioned at an angle in relation to both said pair of generally parallel opposing side edge portions and said end portion;
wherein each of said pair of generally parallel oppositely facing guide members has an inwardly angled edge portion configured for contacting one of said sample cartridge upper corner portions when the sample cartridge is inserted therebetween;
wherein said XYZ stage further comprises: a terminal block member on said planar stage surface portion and positioned to contact the sample cartridge end portion when the sample cartridge is inserted between said pair of generally parallel oppositely facing guide members; and
wherein said locking member on said planar stage surface portion is positioned to press against the sample cartridge locking edge portion at an angle in relation to both said pair of generally parallel opposing side edge portions and said end portion when said sample cartridge is inserted between said pair of generally parallel oppositely facing guide members and in contact with said terminal block member,
so that pressure is exerted by said locking member through said sample cartridge edge portion against both said terminal block member and one of said pair of generally parallel oppositely facing guide members, whereby the sample cartridge is removably locked in place on the XYZ stage in all three of said X, Y and Z planes.
2. The XYZ stage assembly of claim 1 , wherein said sample cartridge is further removably locked in place on the XYZ stage in an X, Y, and Z axes of rotation.