Placental tissue grafts
View Patent ↗A method for preparing placenta membrane tissue grafts for medical use, includes obtaining a placenta from a subject, cleaning the placenta, separating the chorion tissue from the amniotic membrane, mounting a selected layer of either the chorion tissue or the amniotic membrane onto a drying fixture, dehydrating the selected layer on the drying fixture, and cutting the selected layer into a plurality of tissue grafts. Preferably, the drying fixture includes grooves or raised edges that define the outer contours of each desired tissue graft, after they are cut, and further includes raised or indented logos that emboss the middle area of the tissue grafts during dehydration and that enables an end user to distinguish the top from the bottom side of the graft. The grafts are comprised of single layers of amnion or chorion, multiple layers of amnion or chorion, or multiple layers of a combination of amnion and chorion.
1. A method for preparing a dehydrated tissue graft, comprising the steps:
a) obtaining a placenta comprising an amnion and a chorion;
b) separating the amnion and the chorion from the placenta;
c) washing and cleaning the amnion and/or the chorion;
d) placing a first layer of amnion or chorion on a drying fixture;
e) adding at least one additional layer of amnion or chorion to the first layer; and
f) dehydrating the tissue graft on the drying fixture, wherein the drying fixture comprises a receiving surface, said surface containing a raised or indented asymmetric texture within a product space which has an otherwise smooth surface;
wherein when at least one layer is amnion, said method does not substantially remove all of the epithelial cells on at least one layer of amnion.
2. The method of claim 1 , wherein after step (b) but prior to step (c), soaking the amnion in an antibiotic solution and then substantially removing the antibiotic solution.
3. The method of claim 1 , wherein the tissue graft comprises an amnion.
4. The method of claim 1 , wherein the tissue graft comprises a chorion.
5. The method of claim 1 , wherein the tissue graft comprises an amnion and a chorion.
6. The method of claim 1 , wherein the washing step includes contacting the separated amnion with a hypertonic saline solution, wherein the hypertonic saline solution comprises a NaCl concentration from about 10% to about 30%.
7. The method of claim 1 , wherein the dehydrating step comprises drying the placental tissue under conditions wherein the placental tissue is retained on the drying surface such that the portion of the placental tissue placed within the product space molds around the texture thereby creating an asymmetric label on the placental tissue, whereby the orientation of the placental tissue is visibly distinguishable by the asymmetric label.
8. The method of claim 7 , wherein said label is an embossment.
9. The method of claim 7 , wherein said label is a logo, a design, a name, or text.