Control of conductivity in anaerobic fermentation
View Patent ↗Process are provided which are effective for controlling medium conductivity during fermentation of a CO-containing gaseous substrate while providing an STY of about 10 g ethanol/(L·day) or more. The process includes balancing medium conductivity, specific carbon uptake or cell density levels.
1. A process for fermenting a CO-containing gaseous substrate to produce ethanol comprising:
providing a CO-containing gaseous substrate to a fermentation medium;
contacting the resultant CO-containing fermentation medium with one or more acetogenic bacteria;
determining a specific carbon uptake of the CO-containing gaseous substrate by the one or more acetogenic bacteria in the CO-containing fermentation medium and measuring the CO-containing fermentation medium conductivity;
providing the CO-containing gaseous substrate to the CO-containing fermentation medium comprising the one or more acetogenic bacteria in an amount effective for maintaining a conductivity of 4 to 30 mS/cm and where the SCU of the one or more acetogenic bacteria is 0.0001 to 3, wherein SCU is the rate of specific carbon uptake by the one or more acetogenic bacteria in the CO-containing fermentation medium, measured as mmole/minute/gram dry cells.
2. The process of claim 1 wherein the process is effective for maintaining a space time yield (STY) of 10 g ethanol/L-day or more.
3. The fermentation process of claim 1 wherein the CO-containing gaseous substrate comprises CO 2 and has a CO/CO 2 ratio of 0.75 or more.
4. The fermentation process of claim 1 wherein the one or more acetogenic bacteria is selected from the group consisting of Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Blautia producta, Butyribacterium meltylotrophicum, Caldanaerobacter subterraneous, Caldanaerobacter subterraneous pacificus, Carboxydothermus trydrogenoformans, Clostridium aceticum, Clostridium acetobutylicum, Clostridium acetobutylicum P262 (DSM 19630 of DSMZ Germany), Clostridium autoethanogenum (DSM 19630 of DSMZ Germany), Clostridium autoethanogenum (DSM 10061 of DSMZ Germany), Clostridium autoethanogenum (DSM 23693 of DSMZ Germany), Clostridium autoethanogenum (DSM 24138 of DSMZ Germany), Clostridium carboxidivorans P7 (ATCC FrA-7827), Clostridium coskatii (ATCC PTA-10522), Clostridium drakei, Clostridium ljungdahlii PETC (ATCC 49587), Clostridium ljungdahlii ERI2 (ATCC 55380), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii 0-52 (ATCC 55889), Clostridium magnum, Clostridium pasteurianum (DSM 525 of DSMZ Germany), Clostridium ragsdali P11 (ATCC BAA-622), Clostridium scatologenes, Clostridium thermoaceticum, Clostridium ultunense, Desulfotomaculum kuznetsovii, Eubacterium limosum, Geobacter sulfiurreducens, Methanosarcina acetiorans, Methanosarcina barkeri, Morrella thermoacetica, Morrella thermoautotrophica, Oxobacter pfennigii, Peptostreptococcus productus, Ruminococcus productus, Thermoanaerobactr kivui , and mixtures thereof.
5. The process according to claim 1 wherein the CO-containing fermentation medium includes ammonium hydroxide as a nitrogen source.