IP Library › Patent Application 14293362
Patent Application
App. No. 14/293,362

MICROORGANISMS AND PROCESSES FOR THE PRODUCTION OF ISOPRENE

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Quick Facts
Patent No.
US None
App. No.
14/293,362
Abstract

The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.

Claims (14)

1 . A method of producing isoprene, the method comprising the steps of:

a) optionally converting 3-methyl-2-buten-1-ol to 2-methyl-3-buten-2-ol using a 2-methyl-3-buten-2-ol isomerase enzyme; and

b) converting 2-methyl-3-buten-2-ol to isoprene using a 2-methyl-3-buten-2-ol dehydratase enzyme.

2 . The method of claim 1 , wherein the 2-methyl-3-buten-2-ol isomerase enzyme is expressed by an exogenous nucleic acid in a microbial organism.

3 . The method of claim 1 , wherein the 2-methyl-3-buten-2-ol dehydratase enzyme is expressed by an exogenous nucleic acid in a microbial organism.

4 . The method of claim 1 , wherein the 2-methyl-3-buten-2-ol dehydratase is a bi-functional enzyme further comprising 2-methyl-3-buten-2-ol isomerase activity.

5 . The method of claim 4 , wherein the 2-methyl-3-buten-2-ol dehydratase is a linalool dehydratase-isomerase.

6 . The method of claim 5 , wherein the linalool dehydratase-isomerase is derived from Castellaniella defragrans.

7 . The method of claim 5 , wherein the linalool dehydratase-isomerase is expressed by an exogenous nucleic acid in microbial organism.

8 . The method of claim 2 , wherein the microbial organism is selected from bacteria, archaea, eubacteria, yeast and fungi.

9 . The method of claim 8 , wherein the microbial organism is an Escherichia coli.

10 . The method of claim 3 , wherein the microbial organism is selected from bacteria, archaea, eubacteria, yeast and fungi.

11 . The method of claim 10 , wherein the microbial organism is an Escherichia coli.

12 . The method of claim 7 , wherein the microbial organism is an Escherichia coli.