Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
View Patent ↗The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.
1. A method of altering expression of at least one gene product in a eukaryotic cell containing and expressing a DNA molecule having a target sequence and encoding said gene product comprising introducing into said eukaryotic cell an engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) system comprising one or more vectors comprising:
a) a first regulatory element operable in a eukaryotic cell operably linked to at least one nucleotide sequence encoding a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and
b) a second regulatory element operable in a eukaryotic cell operably linked to a nucleotide sequence encoding a Staphylococcus aureus Cas9 protein,
wherein the CRISPR-Cas system further comprises one or more nuclear localization signal(s) (NLS(s)), and components (a) and (b) are located on same or different vectors of the system,
whereby the guide RNA targets the target sequence and the Cas9 protein cleaves the DNA molecule;
the method further comprising inserting DNA into a cleaved strand of the DNA molecule;
whereby expression of the at least one gene product is altered; and, wherein the Cas9 protein and the guide RNA do not naturally occur together.
2. The method of claim 1 wherein the inserting of DNA into a cleaved strand of the DNA molecule comprises homologous recombination.
3. The method of claim 1 wherein the Staphylococcus aureus Cas9 protein comprises one or more mutations, wherein the mutation comprises a mutation at position 10, 762, 840, 854, 863 or 986 with reference to the position numbering of a Streptococcus pyogenes Cas9 protein.
4. The method of claim 1 , wherein the insertion generates an altered transcription or translation gene product that is expressed.
5. The method of claim 1 , wherein the CRISPR-Cas system comprises a trans-activating cr (tracr) sequence.
6. The method of claim 1 , wherein the guide RNA comprises a guide sequence and a tracr sequence.
7. The method of claim 1 , wherein the nucleotide sequence encoding the Staphylococcus aureus Cas9 protein is codon optimized for expression in the eukaryotic cell.
8. The method of claim 1 , wherein the eukaryotic cell is a mammalian cell.
9. The method of claim 1 , wherein the eukaryotic cell is a human cell.
10. The method of claim 6 , wherein the guide sequence and tracr sequence are chimeric.
11. The method of claim 1 , wherein the expression of one or more gene products is decreased.
12. The method of claim 1 , wherein the one or more vectors are viral vectors.
13. The method of claim 12 , wherein the viral vectors are selected from the group consisting of retroviral, lentiviral, adenoviral, adeno-associated and herpes simplex viral vectors.
14. A CRISPR-Cas system-mediated genome targeting method in a eukaryotic cell containing a DNA molecule having a target sequence comprising introducing into said eukaryotic cell an engineered, non-naturally occurring CRISPR-Cas system comprising one or more vectors comprising:
a) a first regulatory element operable in a eukaryotic cell operably linked to at least one nucleotide sequence encoding a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and
b) a second regulatory element operable in a eukaryotic cell operably linked to a nucleotide sequence encoding a Staphylococcus aureus Cas9 protein,
wherein the CRISPR-Cas system further comprises one or more nuclear localization signal(s) (NLS(s)) and components (a) and (b) are located on same or different vectors of the system;
the method further comprising inserting DNA into a cleaved strand of the DNA molecule;
whereby there is CRISPR-Cas system-mediated genome targeting through the CRISPR-Cas system acting as to the DNA molecule comprising the guide RNA directing sequence-specific binding of the CRISPR-Cas system, whereby there is genome editing; and, wherein the Cas9 protein and the guide RNA do not naturally occur together.
15. The method of claim 14 wherein the inserting of DNA into a cleaved strand of the DNA molecule comprises insertion of a homologous recombination (HR) template into cleaved strand of the DNA molecule.
16. The method of claim 14 , wherein expression of two or more gene products is altered by the CRISPR-Cas system-mediated genome targeting.
17. The method of claim 14 , wherein the CRISPR-Cas system comprises a tracr sequence.
18. The method of claim 14 , wherein the nucleotide sequence encoding the Staphylococcus aureus Cas9 protein is codon optimized for expression in the eukaryotic cell.
19. The method of claim 14 , wherein the eukaryotic cell is a mammalian or human cell.
20. The method of claim 14 , wherein expression of one or more gene products is increased by the CRISPR-Cas system-mediated genome targeting.
21. The method of claim 14 , wherein expression of one or more gene products is decreased by the CRISPR-Cas system-mediated genome targeting.
22. The method of claim 14 , wherein the Staphylococcus aureus Cas9 protein comprises one or more mutations, wherein the mutation comprises a mutation at position 10, 762, 840, 854, 863 or 986 with reference to the position numbering of a Streptococcus pyogenes Cas9 protein.
23. The method of claim 14 , wherein the one or more vectors are viral vectors.
24. The method of claim 23 , wherein the one or more viral vectors are selected from the group consisting of retroviral, lentiviral, adenoviral, adeno-associated and herpes simplex viral vectors.
25. An engineered, programmable, non-naturally occurring Type II CRISPR-Cas system comprising a Staphylococcus aureus Cas9 protein, at least one guide RNA that targets and hybridizes to a target sequence of a DNA molecule in a eukaryotic cell, one or more NLS(s), and DNA for insertion into a cleaved strand of the DNA molecule; wherein the Cas9 protein cleaves the DNA molecule, and the DNA for insertion inserts into a cleaved strand of the DNA molecule, whereby the CRISPR-Cas system when introduced into a eukaryotic cell having the DNA molecule provides mediated genome targeting; and, wherein the Cas9 protein and the guide RNA do not naturally occur together.
26. The CRISPR-Cas system of claim 25 wherein the DNA for insertion comprises a homologous recombination (HR) template.
27. The CRISPR-Cas system of claim 25 , wherein the Staphylococcus aureus Cas9 protein comprises one or more mutations, wherein the mutation comprises a mutation at position 10, 762, 840, 854, 863 or 986 with reference to the position numbering of a Streptococcus pyogenes Cas9 protein.
28. The CRISPR-Cas system of claim 25 , wherein a nucleotide sequence encoding the Staphylococcus aureus Cas9 protein is codon optimized for expression in the eukaryotic cell.
29. The method of claim 14 , wherein the guide RNA comprises a guide sequence and a tracr sequence and wherein the guide sequence and tracr sequence are chimeric.
30. The CRISPR-Cas system of claim 25 , wherein the guide RNA comprises a guide sequence and a tracr sequence and wherein the guide sequence and tracr sequence are chimeric.