Covalent tethering of functional groups to proteins and substrates therefor
View Patent ↗A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
1. A fusion polypeptide of a protein of interest and a mutant haloalkane dehalogenase having at least 85% sequence identity to the wild type dehalogenase polypeptide of SEQ ID NO:82 and comprising one to three amino acid substitutions at positions 106, 130, and 272 relative to the wild-type dehalogenase.
2. The fusion polypeptide of claim 1 , wherein the protein of interest is a selectable marker protein, membrane protein, cytosolic protein, nuclear protein, structural protein, an enzyme, an enzyme substrate, a receptor protein, a transporter protein, a transcription factor, a channel protein, a phospho-protein, a kinase, a signaling protein, a metabolic protein, a mitochondrial protein, a receptor associated protein, a nucleic acid binding protein, an extracellular matrix protein, a secreted protein, a receptor ligand, a serum protein, an immunogenic protein, a fluorescent protein, or a protein with reactive cysteine.
3. The fusion polypeptide of claim 1 , wherein said mutant haloalkane dehalogenase is covalently attached to a substrate.
4. The fusion polypeptide of claim 3 , wherein the substrate comprises a functional group.
5. The fusion polypeptide of claim 4 , wherein the functional group is a molecule with one or more properties that facilitate detection or isolation of the substrate with the mutant haloalkane dehalogenase attached covalently thereto.
6. The fusion polypeptide of claim 4 , wherein the functional group is selected from the group consisting of a fluorophore, chromophore, luminophore, nucleic acid, a peptide, a solid support, a radionuclide, a contrast agent, a metal ion chelator, an affinity molecule, a drug, a toxin, a substrate for an enzyme, an inhibitor of an enzyme, a protein ligand, a DNA intercalator, and a crosslinker.
7. The fusion polypeptide of claim 1 , wherein the substitution relative to the wild type dehalogenase polypeptide is one substitution at amino acid position 272.
8. The fusion polypeptide of claim 7 , wherein the amino acid at position 272 is phenylalanine.
9. The fusion polypeptide of claim 1 , further comprising substitutions relative to the wild type dehalogenase polypeptide at one to three of amino acid positions 175, 176 and 273.