Compositions and methods for encapsulating vaccines for the oral vaccination and boostering of fish and other animals
A composition comprising a drug or pharmaceutically active agent and a bioadhesive delivery system provides for the oral delivery of a vaccine to animals, particularly aquatic animals.
1. A composition for oral delivery, comprising one or more drugs constituting in total from about 0.05% to about 10% w/w of the composition, one or more bioadhesive polymers selected from the group consisting of chitosan, dimethyl chitosan, trimethyl chitosan, carboxymethyl chitosan, hyaluronic acid, gum Karaya, cationic guar and mixtures thereof and constituting in total from about 0.05% to about 10% w/w of the composition, and one or more short chain polysaccharides or oligosaccharides constituting in total from about 0.05% to about 30% w/w of the composition, wherein the composition is a crosslinked solid;
wherein the composition further comprises one or more adjuvant compounds.
2. A method of preparing a composition for oral delivery, wherein the composition comprises one or more pharmaceutically active agents constituting in total from about 0.05% to about 10% w/w of the composition, one or more bio-adhesive polymers selected from the group consisting of chitosan, dimethyl chitosan, trimethyl chitosan, carboxymethyl chitosan and mixtures thereof and constituting in total from about 0.05% to about 10% w/w of the composition, and one or more short chain polysaccharides or oligosaccharides constituting in total from about 0.05% to about 30% w/w of the composition, wherein the composition is a crosslinked solid;
wherein said one or more pharmaceutically active agents are selected from the group consisting of immunogenic peptides, immunogenic proteins, intact inactive viral particles, attenuated viral particles, infectious viral particles, intact killed prokaryotes, attenuated prokaryotes, infectious prokaryotes, intact killed protozoans, attenuated protozoans, infectious protozoans, intact killed multicellular pathogens, attenuated multicellular pathogens, infectious multicellular pathogens, recombinant subunit vaccines, recombinant vectors encoding immunogenic proteins, recombinant vectors for delivering and expressing genes encoding immunogenic proteins, and mixtures of any of these, and wherein the composition further comprises one or more adjuvant compounds;
said method comprising:
(i) preparing an aqueous solution comprising said one or more bioadhesive polymers, wherein the pH is such that the bioadhesive polymer(s) is/are soluble;
(ii) combining a short chain polysaccharide or oligosaccharide into the aqueous solution;
(iii) combining the pharmaceutically active agent with an adjuvant compound selected from the group consisting of beta-glucans, squalene and squalane oil in a water in oil emulsion comprising an emulsifier selected from the group consisting of lecithin, polysorbates, sucrose esters of medium and long chain saturated fatty acids, and mixtures thereof;
(iv) adding the solution or emulsion of step (iii) into the product of step (ii); and
(v) precipitating or extruding the product of step (iv) into a cross-linking solution comprising a crosslinking agent to form a crosslinked solid product.
3. The method of claim 2 , wherein the short chain polysaccharide or oligosaccharide is selected from inulin, maltodextrins and cyclodextrins.
4. The method of claim 2 , wherein the composition comprises from about 0.1% to about 10% of the adjuvant compound.
5. The method of claim 2 , wherein the adjuvant compound is selected from beta-glucans.
6. The method of claim 2 , wherein in step (iii) the pharmaceutically active agent is in said water in oil emulsion.
7. The method of claim 2 , wherein the crosslinking agent comprises from about 1% to about 20% of phosphate or carbonate anions.
8. The method of claim 2 , wherein the crosslinking solution further comprises about 1% to 30% of a sugar.
9. The method of claim 2 , wherein the crosslinking solution further comprises about 1% to 30% of an alcohol.
10. The method of claim 2 , wherein the solid product of step (v) is dried.
11. A composition made by the method of claim 2 .
12. A method of orally vaccinating or boostering an animal against a pathogen, comprising the steps of:
dispersing or spraying a liquid mixture comprising the composition of claim 11 and a liquid carrier onto an animal feed; and
delivering the resulting animal feed to the animal at a similar or greater dose of immunogenic agent than is usually provided by injection or immersion.
13. The method of claim 12 , wherein the delivery is repeated.
14. The method of claim 12 , wherein the delivery is repeated every three days for up to ten feeding events to maximize immunogenicity.
15. The composition of claim 11 , wherein the crosslinked solid is combined with a thickening agent selected from the group consisting of methylcellulose, pectin, alginate, xanthan gum, carboxymethyl cellulose and hydroxypropyl cellulose.
16. The composition of claim 15 , further comprising feed pellets upon which the composition is top-coated.
17. The composition of claim 11 , wherein the crosslinked solid is combined with an edible oil.
18. The composition of claim 17 , further comprising feed pellets upon which the composition is top-coated.