Extracellular matrix (ECM) structures for tissue regeneration
View Patent ↗An extracellular matrix (ECM) structure for tissue regeneration, the ECM structure having a a sheet member comprising small intestine submucosa (SIS), the SIS sheet member being folded and laminated proximate the sheet member edge, wherein a folded laminated ECM structure having a cavity therein is formed, the ECM structure further including an ECM composition that is disposed in the ECM structure cavity, the ECM composition including liver basement membrane, urinary bladder submucosa, a mesenchymal stem cell and a growth factor.
1. An extracellular matrix (ECM) structure for tissue regeneration, comprising:
a sheet member comprising first acellular ECM from a first mammalian tissue source, said first mammalian tissue source comprising small intestine submucosa (SIS),
said SIS sheet member having a periphery, an edge that extends around said SIS sheet member periphery, a first end and an opposing second end, said SIS sheet member further including a mid-region that is disposed between said first and second ends,
said SIS sheet member being folded proximate said mid-region, wherein said first end is adjacent said second end, and laminated proximate said edge, wherein a folded laminated ECM structure having a cavity therein is formed,
said folded laminated ECM structure further comprising an ECM composition comprising second acellular ECM from a second mammalian tissue source, third acellular ECM from a third mammalian tissue source, a mesenchymal stem cell and a growth factor,
said second mammalian tissue source comprising liver basement membrane, said third mammalian tissue source comprising urinary bladder submucosa, said growth factor comprising fibroblast growth factor-2 (FGF-2),
said ECM composition being disposed in said folded laminated ECM structure cavity.
2. An extracellular matrix (ECM) structure for tissue regeneration, comprising:
a sheet member comprising first ECM, said first ECM comprising small intestine submucosa (SIS),
said SIS sheet member having a periphery, an edge that extends around said SIS sheet member periphery, a first end and an opposing second end, said SIS sheet member further including a mid-region that is disposed between said first and second ends,
said SIS sheet member being folded proximate said mid-region, wherein said first end is adjacent said second end, and laminated proximate said edge, wherein a folded laminated ECM structure having a cavity therein is formed,
said folded laminated ECM structure further comprising an ECM composition comprising second acellular ECM from a second mammalian tissue source, third acellular ECM from a third mammalian tissue source, a mesenchymal stem cell and a growth factor,
said second mammalian tissue source comprising liver basement membrane, said third mammalian tissue source comprising urinary bladder submucosa, said growth factor comprising a vascular epithelial growth factor (VEGF),
said ECM composition being disposed in said folded laminated ECM structure cavity.
3. An extracellular matrix (ECM) structure for tissue regeneration, comprising:
a sheet member comprising first ECM, said first ECM comprising small intestine submucosa (SIS),
said SIS sheet member having a periphery, an edge that extends around said SIS sheet member periphery, a first end and an opposing second end, said SIS sheet member further including a mid-region that is disposed between said first and second ends,
said SIS sheet member being folded proximate said mid-region, wherein said first end is adjacent said second end, and laminated proximate said edge, wherein a folded laminated ECM structure having a cavity therein is formed,
said folded laminated ECM structure further comprising an ECM composition comprising second acellular ECM from a second mammalian tissue source, third acellular ECM from a third mammalian tissue source, a pluripotent cell and a growth factor,
said second mammalian tissue source comprising liver basement membrane, said third mammalian tissue source comprising urinary bladder submucosa, said growth factor comprising fibroblast growth factor-2 (FGF-2),
said ECM composition being disposed in said folded laminated ECM structure cavity.
4. An extracellular matrix (ECM) structure for tissue regeneration, comprising:
a sheet member comprising first ECM, said first ECM comprising small intestine submucosa (SIS),
said SIS sheet member having a periphery, an edge that extends around said SIS sheet member periphery, a first end and an opposing second end, said SIS sheet member further including a mid-region that is disposed between said first and second ends,
said SIS sheet member being folded proximate said mid-region, wherein said first end is adjacent said second end, and laminated proximate said edge, wherein a folded laminated ECM structure having a cavity therein is formed,
said folded laminated ECM structure further comprising an ECM composition comprising second acellular ECM from a second mammalian tissue source, third acellular ECM from a third mammalian tissue source, a pluripotent cell and a growth factor,
said second mammalian tissue source comprising liver basement membrane, said third mammalian tissue source comprising urinary bladder submucosa, said growth factor comprising a vascular epithelial growth factor (VEGF),
said ECM composition being disposed in said folded laminated ECM structure cavity.