Methods and devices for small volume liquid containment
In one embodiment described herein, a cartridge is provided comprising a cartridge frame; a plurality of diluents each in an expandable container; a plurality of reagents each in an expandable container; and a plurality of mixing vessels comprising empty expandable containers.
1. A cartridge comprising:
a cartridge frame;
a plurality of expandable containers supported by the cartridge frame, each of said containers is expandable from a substantially flat configuration with substantially no interior cavity to a non-flat configuration when filled with liquid therein and collapsible from the non-flat configuration to the substantially flat configuration when said liquid is removed;
wherein the non-flat configuration has a cross-sectional shape defining:
a bulk portion in a first portion of the cross-sectional shape;
a liquid capture portion in a second portion of the cross-sectional shape, wherein the first portion is wider than the second portion;
wherein each of the containers comprises a cover portion defining an access port in fluidic communication with both the bulk portion and the liquid capture portion;
a plurality of reagents, each of said reagents in one of said expandable containers in the non-flat configuration; and
a plurality of mixing vessels comprising empty expandable containers in the substantially flat configuration.
2. The cartridge of claim 1 wherein the containers are each configured to hold about 50 microliters or less of fluid.
3. The cartridge of claim 1 wherein the containers are each configured to have a neutral bias provided by the container in terms of expelling or drawing liquid into the vessel.
4. The cartridge of claim 1 wherein each of the containers comprises at least one space between an outer vessel and an inner vessel that is configured to be pressurized to compress the inner vessel and direct any liquid out of the inner vessel.
5. The cartridge of claim 4 wherein each of the containers comprises at least a portion of the inner vessel is coupled to the outer vessel.
6. The cartridge of claim 1 wherein the liquid capture portion comprises a capillary portion.
7. The cartridge of claim 1 wherein the liquid capture portion comprises a capillary channel.
8. The cartridge of claim 1 wherein the liquid capture portion comprises an open capillary channel.
9. The cartridge of claim 1 wherein the liquid capture portion comprises a liquid wicking channel.
10. The cartridge of claim 1 wherein the liquid capture portion comprises a liquid directing channel.
11. The cartridge of claim 1 wherein the liquid capture portion comprises a meniscus elongation structure.
12. The cartridge of claim 1 wherein the liquid capture portion comprises a meniscus shaping structure.
13. The cartridge of claim 1 wherein the liquid capture portion comprises a surface tension based liquid shaping structure to draw a portion of liquid in fluid communication to extend away from a bulk portion of the liquid.
14. The cartridge of claim 1 wherein there is only a single access port into each of the containers.
15. The cartridge of claim 1 wherein there are multiple access ports into each of the containers.
16. The cartridge of claim 1 wherein lower portions of the bulk portion are coated with a hydrophobic material to encourage flow towards an orifice.
17. The cartridge of claim 1 wherein each of the containers further comprise an inside wall surface material that is wetting or partially wetting to the liquids defined by an advancing contact angle less than 90 degrees.
18. The cartridge of claim 1 wherein each of the containers further comprise a coating on the vessel inside wall surface, particularly the channel, that is wetting or partially wetting to the liquids defined by thean advancing contact angle less than 90 degrees.
19. The cartridge of claim 1 wherein each of the containers comprises a bulk fluid zone and a capillary fluid zone.