Genetic markers associated with drought tolerance in maize
View Patent ↗The presently disclosed subject matter relates to methods and compositions for identifying, selecting, and/or producing drought tolerant maize plants or germplasm. Maize plants or germplasm that have been identified, selected, and/or produced by any of the methods of the presently disclosed subject matter are also provided.
1. A method of producing a maize plant having improved water optimization, the method comprising the steps of:
a) isolating at least one nucleic acid from a maize plant selected from a population of maize plants;
b) detecting by way of amplifying a marker locus or a portion of a marker locus from the nucleic acid of a) and thereby, producing a amplicon diagnostic for at least one allele located on maize chromosome 8 and further located within 5cM to a water optimization locus that is associated with increased water optimization, wherein the water optimization locus comprises ZmDr4, and further wherein ZmDr4 comprises the following alleles corresponding to SEQ ID NO: 7 comprising a deletion at positions 4497-4498; a G at position 4505; a T at position 4609; an A at position 4641; a T at position 4792; a T at position 4836; a C at position 4844; a G at position 4969; a TCC at positions 4979-4981; and an A at position 526;
c) selecting a first maize plant from the population of a) based on the presence of the allele detected in b);
d) crossing the first maize plant with a second maize plant, wherein the second maize plant does not have in its genome the ZmDr4 water optimization locus; and
e) producing a progeny maize plant having improved water optimization from the cross of d) wherein said progeny plant comprises the ZmDR4 locus as well as the allele detected in b).
2. The method of claim 1 , wherein the second maize plant or plant produced in e) is backcrossed one or more times.
3. The method of claim 1 , wherein either the first maize plant or second maize plant is an inbred.
4. The method of claim 1 , wherein the allele is detected with a nucleotide probe or primer comprising any one of SEQ ID NOs: 130; 131; 242-251; or 350-353.