Recurrent gene fusions in cancer
View Patent ↗The present disclosure relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present disclosure relates to gene fusions as diagnostic markers and clinical targets for cancer.
1. A method for detecting the presence of a gene fusion in a sample from a subject comprising
(a) contacting a biological sample from a subject with at least a first gene fusion informative reagent for identification of a SLC45A3-FGFR2 gene fusion having SEQ ID NO: 9, wherein said at least a first gene fusion informative reagent is probe that specifically hybridizes to the SLC45A3-FGFR2 junction of SEQ ID NO: 9, and
(b) detecting hybridization of the probe to the sample, and thus, detecting the presence of said gene fusion in said sample using said reagent.
2. A method for detecting the presence of a gene fusion in a sample from a subject comprising
(a) contacting a biological sample from a subject with at least a first gene fusion informative reagent for identification of a SLC45A3-FGFR2 gene fusion having SEQ ID NO: 9, wherein said at least a first gene fusion informative reagent is a pair of amplification primers that amplify the SLC45A3-FGFR2 junction of SEQ ID NO: 9, and
(b) amplifying a nucleic acid comprising the SLC45A3-FGFR2 junction, and detecting the amplification product comprising the junction of SEQ ID NO: 9, thus detecting the presence of said gene fusion in said sample using said reagent.
3. The method of claim 2 , wherein said pair of amplification primers comprises a first primer that hybridizes to exon 1 of SLC45A3 and a second primer that hybridizes to exon 3b of FGFR2.
4. A method for detecting the presence of a gene fusion in a sample from a subject comprising
(a) contacting a biological sample from a subject with at least a first gene fusion informative reagent for identification of a SLC45A3-FGFR2 gene fusion having SEQ ID NO: 9, wherein said at least a first gene fusion informative reagent is a sequencing primer that binds SEQ ID NO: 9
(b) generating an extension product that includes the fusion junction of SEQ ID NO: 9, and
(c) detecting the extension product comprising the junction of SEQ ID NO: 9, thus detecting the presence of said gene fusion in said sample using said reagent.
5. The method of claim 1 , 2 or 4 , wherein the sample is selected from the group consisting of tissue, blood, plasma, serum, cells and tissues.
6. The method of claim 1 , 2 , or 4 , wherein said reagent is labeled.