Anti-cancer nanoparticle compositions and methods of use
The present invention encompasses a composition capable of delivering and expressing a nucleic acid encoding UDP-Glucuronosyltransferases, p53 or a combination thereof into a cell, and methods for treating tumors.
1. An isolated pancreatic tumor cell, wherein the tumor cell comprises at least one nucleic acid attached to a nanoparticle, wherein the at least one nucleic acid is a nucleic acid encoding UGT2B15, and the nanoparticle is selected from the group consisting of a carbon nanotube, carbon nanorod, and carbon nanosphere, such that the nucleic acid is expressed within the pancreatic tumor cell.
2. The composition of claim 1 , wherein the nanotube is selected from the group consisting of a single-wall carbon nanotube, a double-wall carbon nanotube, and a multi-wall carbon nanotube.
3. The composition of claim 1 , wherein the nanoparticle is bio-degradable and/or bio-compatible.
4. The composition of claim 1 , wherein the nucleic acid is attached to the nanoparticle using a method selected from the group consisting of covalent bonding, hydrogen bonding, click attachment, and physical attachment.
5. The composition of claim 1 , wherein the nucleic acid is non-covalently attached.
6. The composition of claim 1 , wherein the composition further comprises a targeting agent.
7. An ex vivo pancreatic tumor cell, wherein the tumor cell comprises at least one nucleic acid attached to a nanoparticle, wherein the at least one nucleic acid is a nucleic acid encoding UGT2B15, and the nanoparticle is selected from the group consisting of a carbon nanotube, carbon nanorod, and carbon nanosphere, such that the nucleic acid is expressed within the pancreatic tumor cell.
8. The composition of claim 7 , wherein the nanotube is selected from the group consisting of a single-wall carbon nanotube, a double-wall carbon nanotube, and a multi-wall carbon nanotube.
9. The composition of claim 7 , wherein the nanoparticle is bio-degradable and/or bio-compatible.
10. The composition of claim 7 , wherein the nucleic acid is attached to the nanoparticle using a method selected from the group consisting of covalent bonding, hydrogen bonding, click attachment, and physical attachment.
11. The composition of claim 7 , wherein the nucleic acid is non-covalently attached.
12. The composition of claim 7 , wherein the composition further comprises a targeting agent.