Capture probes for use in detecting the presence of
View Patent ↗Oligomers useful for determining the presence of Trichomonas vaginalis in a test sample.
1. A set of capture probes comprising:
a first capture probe consisting of: (i) a target-complementary sequence, the base sequence of which is selected from the group consisting of the base sequences of SEQ ID NO: 90 and SEQ ID NO: 91; and (ii) a non-complementary sequence comprising a homopolymer tail, wherein the non-complementary sequence does not stably bind to a target nucleic acid derived from Trichomonas vaginalis when the target-complementary sequence is stably hybridized to the target nucleic acid; and
a second capture probe consisting of: (i) a target-complementary sequence, the base sequence of which is selected from the group consisting of SEQ ID NO: 55 and SEQ ID NO: 56; and (ii) a non-complementary sequence comprising a homopolymer tail, wherein the non-complementary sequence does not stably bind to a target nucleic acid derived from Trichomonas vaginalis when the target-complementary sequence is stably hybridized to the target nucleic acid.
2. The set of capture probes of claim 1 , wherein the non-complementary sequence of each of the first and second capture probes comprises a spacer region for joining the target-complementary sequence to the homopolymer tail.
3. The set of capture probes of claim 1 , wherein the homopolymer tail of each of the first and second capture probes is a poly dA sequence.
4. The set of capture probes of claim 2 , wherein the homopolymer tail of each of the first and second capture probes is a poly dA sequence, and wherein the spacer region of each of the first and second capture probes is a poly dT sequence.
5. The set of capture probes of claim 1 , wherein the target-complementary sequence of each of the first and second capture probes comprises at least one ribonucleoside having 2′-O-methyl substitution to the ribofuranosyl moiety.
6. The set of capture probes of claim 2 , wherein the non-complementary sequence of each of the first and second capture probes consists of the homopolymer tail and the spacer region.
7. The set of capture probes of claim 3 , wherein the non-complementary sequence of each of the first and second capture probes consists of the homopolymer tail.
8. The set of capture probes of claim 4 , wherein the non-complementary sequence of each of the first and second probes consists of the homopolymer tail and the spacer region.
9. The set of capture probes of claim 1 , wherein the non-complementary sequence of each of the first and second probes consists of the sequence of SEQ ID NO:60.