Detection of hydrogen peroxide

1. A compound according to Formula (II):

wherein

R 11 is boronic acid or a borate ester;

R 2 is —(CH 2 ) n -T or C 1-5 alkyl;

R 6 is selected from the group consisting of —H, —OH, —NH 2 —OC(O)R or —OCH 2 OC(O)R;

R 8 is selected from the group consisting of

 H or lower cycloalkyl;

wherein R 3 and R 4 are both H or both C 1-2 alkyl;

n is 0 to 3;

each R is independently a C 1-2 alkyl;

T is aryl, heteroaryl, substituted aryl, substituted heteroaryl or cycloalkyl;

L 2 is a linker;

and the dashed bonds indicate the presence of an optional ring which may be saturated or unsaturated.

2. A compound according to claim 1 , wherein L 2 is

A is —C 6 (R 10 ) 4 — or —(CR 21 —CR 21 ) n — or —O—C 6 (R 10 ) 4 — or —S—C 6 (R 10 ) 4 — or —NR′—C 6 (R 10 ) 4 — or a direct bond; R′ is H or C 1-4 alkyl; each R 3 is independently halo, H, C 1-4 alkyl, C 1-4 hydroxyalkyl, or C 1-4 alkylcarboxylic acid; each R 10 is independently H, halo, CH 3 , OCH 3 , or NO 2 ; each R 21 is independently H or CH 3 ; n is 1 or 2; and X is selected from a direct bond, —C(O)—, and —C(O)NR 22 , where R 22 is H or C 1-4 alkyl.

3. A compound according to claim 1 , wherein

R 11 is

 and

each R 12 and R 13 is independently selected from H, C 1-4 alkyl, CF 3 , phenyl or substituted phenyl; or R 12 and R 13 together can be an alkyl ring having from 3-7 carbons or can be replaced by a fused 6-membered aromatic ring.

4. A compound according to claim 1 , wherein

R 11 is

 and

each R 14 , R 15 and R 16 is independently selected from H, C 1-4 alkyl, CF 3 , phenyl and substituted phenyl; or both R 15 together can form an alkyl ring having from 3-7 carbons; R 14 and R 15 together or R 15 and R 16 together can be an alkyl ring having from 3-7 carbon atoms or can be replaced by a 6-membered aromatic ring.

5. A compound according to claim 1 , wherein:

R 2 is

 and X is O or S.

6. A compound according to claim 1 , wherein R 2 is C 2-5 straight-chain alkyl.

7. A compound according to claim 1 , wherein R 8 is

lower cycloalkyl or H, wherein R 3 and R 4 are both H or C 1-2 alkyl.

8. A compound according to claim 1 , wherein, R 8 is benzyl.

9. A method of detecting hydrogen peroxide in a cell comprising:

(a) contacting cells with a compound according to claim 1 ;

(b) adding a luciferase reaction mixture to the contacted cells; and

(c) measuring bioluminescence thereby detecting the presence of hydrogen peroxide in the cell.

10. The method of claim 9 , wherein the cell is in an animal or in a sample.

11. A method of detecting hydrogen peroxide in a cell comprising:

(a) contacting cells with a compound according to claim 1 in a first reaction vessel to form an incubation mixture;

(b) transferring at least a portion of the incubation mixture to a second reaction vessel;

(c) adding a luciferase reaction mixture to the second reaction vessel; and

(d) measuring bioluminescence thereby detecting the presence of hydrogen peroxide in the cell.

12. The method of claim 11 , wherein the cell is in an animal or in a sample.

13. A method of determining the effect of a test compound or test condition on the presence or amount of hydrogen peroxide in a sample comprising:

a) treating a sample with a test compound or test condition,

b) contacting the sample from a) with a compound according to claim 1 ;

c) adding a luciferase reaction mixture to the sample;

d) measuring bioluminescence.

14. The method of claim 13 , wherein the sample comprises a cell, cell medium or physiological sample from an animal.

15. A kit comprising a compound according to claim 1 .

16. A compound of formula