Method of producing a differentiated mammalian cell comprising culturing a single mammalian blastomere
View Patent ↗The present invention relates generally to the field of somatic cell nuclear transfer (SCNT) and to the creation of cloned animals and cells. The disclosure relates to a method of cloning a mammal, obtaining pluripotent cells such as embryonic stem cells, or for reprogramming a mammalian cell using an oocyte and a fertilized embryo.
1. A method of producing a differentiated mammalian cell, the method comprising
culturing a single mammalian blastomere obtained from a mammalian embryo in the presence of blastocyst medium and about 5-10 μg/ml laminin, and in the absence of previously-derived pluripotent stem cells, until embryonic stem (ES) cells are produced, and
culturing the ES cells under conditions that induce their differentiation into a desired differentiated cell.
2. The method of claim 1 , wherein the blastomere is a human blastomere.
3. The method of claim 1 , wherein the differentiated mammalian cell is a mesodermal cell.
4. The method of claim 1 , wherein the differentiated mammalian cell is an endodermal cell.
5. The method of claim 1 , wherein the differentiated mammalian cell is an ectodermal cell.
6. The method of claim 1 , wherein the differentiated mammalian cell is a retinal pigmented epithelial (RPE) cell, a hematopoietic cell, a red blood cell, a platelet, a pancreatic beta cell, a skin cell, a cardiomyocyte, a smooth muscle cell, an endothelial cell, a hepatocyte, a neuron, a glia cell, a skeletal muscle cell, or a vascular cell.
7. The method of claim 1 , wherein the differentiated mammalian cell is an RPE cell.