Isolated polypeptide of the toxin A and toxin B proteins of
View Patent ↗This present invention provides C-TAB.G5 and C-TAB.G5.1 isolated polypeptides comprising the receptor binding domains of C. difficile toxin A and toxin B as set forth in the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 4. The C-TAB.G5 and C-TAB.G5.1 isolated polypeptides may be used to neutralize toxic effects of C. difficile toxin A and/or toxin B.
1. A polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 4.
2. The polypeptide of claim 1 , wherein the polypeptide is isolated.
3. A diagnostic kit for detecting a C. difficile infection in a subject comprising the polypeptide of claim 1 .
4. A pharmaceutical composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable carrier or excipient.
5. The composition of claim 4 , further comprising an adjuvant.
6. The composition of claim 5 , wherein the adjuvant comprises aluminum hydroxide.
7. The composition of claim 4 , further comprising an additional antigen.
8. The composition of claim 1 , further comprising a therapeutic agent selected from the group consisting of anesthetics, analgesics, anti-inflammatories, steroids, antibiotics, antiarthritics, anorectics, antihistamines and antineoplastics.