Single-use mixing and bioreactor systems
View Patent ↗Disclosed are magnetic agitation mixing systems for use with flexible container reaction vessels. In one aspect of the invention, the orientation of magnetic coupling between the impeller magnets and the external driver magnets is modified such that the coupling is neither strictly axial nor strictly radial. Also disclosed are “receiver-less” retainer configurations whereby the single-use container need not have a rigid base that defines a cup or post to engage of rotatable agitator.
1. A mixing system comprising:
a flexible bag ( 128 ) for containing a fluid;
a magnetic agitator ( 142 ) disposable in the bag ( 128 ) for mixing the fluid, the agitator ( 142 ) configured to rotate about an axis of rotation when magnetically coupled to an external magnetic drive system ( 121 );
wherein the flexible bag is configured to be supported by a bag support structure ( 104 ) including a bag support structure wall ( 106 ) that at least partially surrounds, supports or contains the bag ( 128 ) during use and the flexible bag ( 128 ) is further adapted for deformation by a shaped portion of the bag support structure ( 104 ) to define a retainer for the agitator ( 142 ) within the bag ( 128 ) when the agitator ( 142 ) is magnetically coupled to the external magnetic drive system ( 121 );
wherein the flexible bag is devoid of any rigid portion for receiving and holding the agitator in position within the flexible bag.
2. The mixing system of claim 1 , wherein the shaped portion of the bag support structure ( 104 ) is at least one of a cavity, ring-like protuberance or protrusion ( 500 ), groove ( 600 ) or a post for retaining the flexible bag ( 128 ) and the fluid-agitating agitator.
3. The mixing system of claim 1 , wherein the shaped portion of the bag support structure ( 104 ) is an annular groove ( 600 ) in an inner wall of the structure ( 104 ) and the agitator ( 142 ) further comprises a supporting structure ( 210 ) that at least partially surrounds the rotatable agitator ( 142 ) and fits within the groove ( 600 ).
4. The mixing system of claim 1 , wherein the shaped portion of the bag support structure ( 142 ) is an annular protrusion ( 500 ) in an inner wall of the support structure ( 104 ) and the agitator further comprises a supporting structure ( 210 ) that fits against an inner side of the protrusion ( 500 ).