NEW A-PRP MEDICAL DEVICE & TISSUE ENGINEERING COMPOSITION, MANUFACTURING MACHINES AND PROCESS

1 - 35 . (canceled)

36 . A composition comprising hyaluronic acid and either i) an anticoagulant or ii) a cell selector gel or iii) a combination thereof, wherein said composition optionally further comprises whole blood, bone marrow, bone marrow concentrate, a platelet concentrate and/or platelet rich plasma.

37 . The composition according to claim 36 , wherein said composition comprises:

a) hyaluronic acid of about 1400 KDa to about 1600 KDa at about 1.8% to about 2.2% concentration; or

b) hyaluronic acid of about 4000 KDa or above 4000 KDa at about 1.8% to about 2.2% concentration; or

c) at least one low molecular weight hyaluronic acid and at least one high molecular weight hyaluronic acid, wherein:

said low molecular weight hyaluronic acid is less than 600 KDa or about 600 KDa; and

said high molecular weight hyaluronic acid is about 4000 KDa or above 4000 KDa.

38 . A composition comprising hyaluronic acid and whole blood, bone marrow, bone marrow concentrate, a platelet concentrate and/or platelet rich plasma, wherein said hyaluronic acid composition comprises or consists of:

a) hyaluronic acid of about 1400 KDa to about 1600 KDa at about 1.8% to about 2.2% concentration; or

b) hyaluronic acid of about 4000 KDa or above 4000 KDa at about 1.8% to about 2.2% concentration; or

c) at least one low molecular weight hyaluronic acid and at least one high molecular weight hyaluronic acid, wherein:

said low molecular weight hyaluronic acid is less than 600 KDa or about 600 KDa; and

said high molecular weight hyaluronic acid is about 4000 KDa or above 4000 KDa.

39 . The composition according to claim 36 , said composition further comprising:

a) chitosan, fibroin and/or fibroin-silk proteins, and/or

ii) a coagulation activator, thrombin serum, tricalcium phosphate (TCP), a bone substitute, hyaluronic acid composition, calcium gluconate, calcium saccharate, chitosan, fibroins, silk-fibroin proteins, growth factors, mannitol, collagen, albumin, ascorbic acid, cream, fat cells, fat tissue, bone marrow concentrate, lubricin, cd-gelatin, botulinum toxin and/or one or more cell extracts, and/or

ii) analgesic compounds, antibacterial compounds, antibiotics, antifungal compounds, anti-inflammatories, antiparasitic compounds, antiviral compounds, enzymes, enzyme inhibitors, glycoproteins, growth factors, lymphokines, cytokines, hormones, steroids, glucocorticosteroids, immunomodulators, immunoglobulins, minerals, neuroleptics, proteins, peptides, lipoproteins, tumoricidal compounds, tumorstatic compounds, toxins or vitamins.

40 . The composition according to claim 38 , said composition further comprising:

i) chitosan, fibroin and/or fibroin-silk proteins, and/or

ii) a coagulation activator, thrombin serum, tricalcium phosphate (TCP), a bone substitute, hyaluronic acid composition, calcium gluconate, calcium saccharate, chitosan, fibroins, silk-fibroin proteins, growth factors, mannitol, collagen, albumin, ascorbic acid, cream, fat cells, fat tissue, bone marrow concentrate, lubricin, cd-gelatin, botulinum toxin and/or one or more cell extracts, and/or

ii) analgesic compounds, antibacterial compounds, antibiotics, antifungal compounds, anti-inflammatories, antiparasitic compounds, antiviral compounds, enzymes, enzyme inhibitors, glycoproteins, growth factors, lymphokines, cytokines, hormones, steroids, glucocorticosteroids, immunomodulators, immunoglobulins, minerals, neuroleptics, proteins, peptides, lipoproteins, tumoricidal compounds, tumorstatic compounds, toxins or vitamins.

41 . A device or container comprising a composition according to claim 36 .

42 . The device according to claim 41 , wherein said container or device is a tube or a syringe.

43 . The device according to claim 41 , wherein said device is steam sterilized.

44 . A device or container comprising a composition according to claim 38 .

45 . The device according to claim 44 , wherein said container or device is a tube or a syringe.

46 . A medical device or kit comprising a device according to claim 41 .

47 . A method for the preparation of a wound healant composition or tissue healant composition comprising a platelet concentrate composition or platelet-rich plasma composition in combination with hyaluronic acid, comprising the steps of:

a) centrifuging whole blood in a device according to claim 46 ;

b) optionally removing platelet poor plasma;

c) optionally homogenizing the resulting composition and/or re-suspending the cellular deposit in the supernatant;

d) collecting said wound healant or tissue healant composition comprising a platelet concentrate composition or platelet-rich plasma composition in combination with hyaluronic acid;

e) optionally further mixing said wound healant or tissue healant composition; and

f) optionally further combining said wound healant or tissue healant composition with at least one additional substance and/or one or more cell extracts.

48 . A wound healant or tissue healant composition prepared according to the method of claim 47 .

49 . A composition comprising at least one cell extract and a composition according to claim 36 .

50 . A composition comprising at least one cell extract and a composition according to claim 38 .

51 . A wound healant composition or tissue healant composition comprising the composition according to claim 48 and a coagulation activator, thrombin serum, tricalcium phosphate (TCP), a bone substitute, hyaluronic acid composition, calcium gluconate, calcium saccharate, chitosan, fibroin, fibroin-silk proteins, growth factors, mannitol, collagen, albumin, ascorbic acid, cream, fat cells, fat tissue, bone marrow concentrate, lubricin, cd-gelatin, botulinum toxin and/or one or more cell extracts, selected from an extract of keratinocytes, bone marrow, fibroblasts, periosteum or corneal cells, melanocytes and Langheran's cell; fat cells, muscle cells; osteoblasts; chondrocytes; umbilical cord cells; stem cells, mesenchymal stem cells (MSCs), preadipocytes, pre-endothelial cells, Schwann cells or Achilles tendon cells.

52 . A method of treating a subject comprising the administration of a composition to a subject, wherein said composition comprises or consists of:

a) a composition according to claim 36 , or

b) a composition comprising hyaluronic acid and an anticoagulant, wherein said composition optionally comprises a cell selector gel and/or whole blood, a platelet concentrate and/or platelet rich plasma; or

c) a composition comprising hyaluronic acid, an anticoagulant, a cell extract, a platelet concentrate and/or platelet rich plasma.

53 . The method according to claim 52 , wherein said subject is a human, mammal or animal and is treated for:

(i) cellular regeneration or tissue adhesion;

(ii) promoting wound healing or tissue healing and/or sealing and/or regeneration of a tissue and/or a cartilage and/or a bone and/or a nerve in a wound or tissue of a human or animal;

(iii) inducing periodontal regeneration in a wound or a periodontal defect of a mammal with periodontal disease or other condition requiring periodontal regeneration;

(iv) ligament and/or cartilage reconstitution;

(v) promoting skin regeneration in a scar or a wrinkle;

(vi) increasing adipose tissue volume in a mammal with a dermal fat graft or other condition requiring adipose tissue regeneration;

(vii) inducing myocardial regeneration in a mammal with myocardial deficiency or other condition requiring myocardial regeneration tissue regeneration;

(viii) inducing conical regeneration in a mammal with corneal deficiency or other condition requiring corneal regeneration;

(ix) inducing articular or cartilage regeneration in a mammal with articular or cartilage deficiency or other condition requiring articular or cartilage tissue regeneration;

(x) promoting skin regeneration in a scar, a wrinkle or a fat deficiency from human or lower animal;

(xi) inducing peripheral nerve regeneration in a mammal with peripheral nerve damage, nerve suture or spinal cord injury or other condition requiring peripheral nerve regeneration;

(xii) inducing bone regeneration in a mammal with bone damage, bone deficiency or other condition requiring bone regeneration;

(xiii) injections for orthopedic or injections for esthetic;

(xiv) regeneration and/or rejuvenation of skin tissues or treatment of skin damage or in the reduction of pain associated with skin and tissue regeneration;

(xv) wound or tissue healing or regeneration treatments;

(xvi) cardiac regeneration;

(xvii) treatment of bone, cartilage and articular disorders;

(xviii) treatment of corneal disorders;

(xix) treatment of peripheral nerve damage, nerve suture and spinal cord injury, diabetic wounds, large vascular wounds; or

(xx) treatment of articulations or muscular lesions.

54 . The method according to claim 53 , wherein said composition is administered for the regeneration and/or rejuvenation of skin tissues and said composition is administered as an anti-aging agent or skin repairing agent, scar repairing agent, lipoatrophy repairing agent, a wrinkle filling and/or repairing agent, esthetic preparation, aging management, volume corrector and/or hair stimulator.

55 . The method according to claim 52 , wherein said composition is administered as:

(a) an eyewash; or

(b) a mask post laser, post peeling or monotherapy, for glitter, gloss, brilliance or brightness.

56 . A method of treating a subject comprising the administration of a composition to a subject, wherein said composition comprises or consists of:

a) a composition according to claim 38 , or

b) a composition comprising hyaluronic acid and an anticoagulant, wherein said composition optionally comprises a cell selector gel and/or whole blood, a platelet concentrate and/or platelet rich plasma; or

c) a composition comprising hyaluronic acid, an anticoagulant, a cell extract, a platelet concentrate and/or platelet rich plasma.

57 . A composition comprising hyaluronic acid and:

a) whole blood,

b) a platelet concentrate; or

c) platelet rich plasma.

58 . A device comprising:

i) the composition of claim 57 , or

ii) hyaluronic acid and an anticoagulant, or

iii) hyaluronic acid and a cell selector gel.

59 . The device according to claim 58 , wherein said device is a tube or syringe.