Sample preservation method and sample preservation substrate
View Patent ↗The invention discloses a cellulose or glass fiber paper for preservation of biological samples, which comprises 4-30 wt % of a hydrophilic branched carbohydrate polymer. It also discloses a method for preservation of biological samples by applying and drying them on the paper.
1. A method for preserving a biological sample, the method comprising:
a) providing a paper having a surface weight of 40-800 g/m 2 , the paper comprising cellulose fibers or glass fibers, and further comprising 4-30 percentage by weight (“wt %”) of a hydrophilic branched carbohydrate polymer in the paper wherein the carbohydrate polymer is covalently bound to the cellulose fibers or glass fibers, and wherein water extractables in the paper are 0.1-25 wt %;
b) applying the biological sample on the paper and forming a sample spot with the applied sample absorbed on the paper; and
c) drying the paper with the applied sample absorbed thereon, thereby preserving the biological sample.
2. The method of claim 1 , wherein the biological sample is a whole blood sample.
3. The method of claim 1 , further comprising storing the dried paper for at least one week.
4. The method of claim 1 , wherein the paper comprises 10-25 wt % of the carbohydrate polymer.
5. The method of claim 1 , wherein a residual moisture content of the dried sample paper is less than 10%.
6. The method of claim 1 , wherein water extractables in the paper are 0.1-5 wt %.
7. The method of claim 1 , wherein the water extractables in the paper are 3-20 wt %.
8. The method of claim 1 , wherein the carbohydrate polymer has an average molecular weight of about 70-400 kDa.
9. The method of claim 1 , wherein a 10 wt % water solution of the carbohydrate polymer has a viscosity of 1-10 mPas at 20° C.
10. The method of claim 1 , wherein the carbohydrate polymer is a polysucrose.
11. The method of claim 1 , further comprising storing the dried paper and extracting the biological sample from the dried paper after storage, the extracted biological sample further comprising a protein preserved in a biologically active state.
12. The method of claim 11 , wherein the protein is a storage-sensitive protein.
13. The method of claim 11 , further comprising recovering the protein in the biologically active state with a recovery of at least 60%.
14. The method of claim 1 , further comprising storing the dried paper at a storage temperature of at least 0° C.
15. The method of claim 14 , wherein the storage temperature is 0-40° C.
16. The method of claim 1 , wherein the paper further comprises 5-300 micromole/g negatively or positively charged groups, wherein the negatively or positively charged groups are covalently bound to at least one of the cellulose fibers, the glass fibers, or the carbohydrate polymer.
17. The method of claim 16 , wherein the negatively charged group is a carboxylate group.
18. The method of claim 16 , wherein the positively charged group is an amine group.
19. A method for preserving a biological sample, the method comprising:
a) providing a paper comprising cellulose fibers or glass fibers, and further comprising 4-30 percentage by weight (“wt %”) of a hydrophilic branched carbohydrate polymer in the paper wherein the carbohydrate polymer is covalently bound to the cellulose fibers or glass fibers, the carbohydrate polymer is a polysucrose, and water extractables in the paper are 0.1-25 wt %;
b) applying the biological sample on the paper and forming a sample spot with the applied sample absorbed on the paper; and
c) drying the paper with the applied sample absorbed thereon, thereby preserving the biological sample.