Liquid medium and sample vial for use in a method for detecting cancerous cells in a cell sample
The current invention concerns a method for determining the presence of a (pre)cancerous cell in a liquid cell sample, comprising the steps of: —suspending and preserving a cell sample obtained from a subject in a sample vial comprising a liquid medium, said liquid medium comprises means for labeling cells or epitope(s) on or in said cells; —obtaining data from said labelled liquid cell sample; and —determining the presence of said (pre)cancerous cells based on said obtained data; characterized in that said data comprises morphological data and biomarker data. In a further aspect, the invention relates to a liquid medium for fixing, preserving and labeling cells in a cell sample and a sample vial specifically designed to be used in conjunction with the current method.
1. A method for obtaining data from a cell sample containing cells from a subject at risk of cancer or pre-cancer, comprising the steps of:
suspending and preserving the cell sample in a sample vial comprising a liquid medium, said liquid medium comprising a component configured to label cells or epitope(s) on or in said cells; and
obtaining data from said labelled liquid cell sample by digital holographic microscopy on the suspended cells of the cell sample in the sample vial,
wherein said data comprises morphological data and biomarker data, said morphological data comprising optical nuclear height, nuclear volume, nuclear volume variability, or any combination thereof.
2. The method according to claim 1 , wherein said morphological data and biomarker data are simultaneously obtained.
3. The method according to claim 1 , wherein said sample is solubilized immediately after obtainment.
4. The method according to claim 1 , wherein said morphological data further comprises cell nucleus diameter, chromatin texture, cell size, percent nucleation, cell form, cell quantity, nuclear size, nuclear size variability, chromatin texture, or any combination thereof.
5. The method according to claim 1 , wherein said liquid cell sample comprises cervical cells.
6. The method according to claim 1 , wherein said component configured to label cells or epitopes on or in said cells comprises a labelled biomarker probe for obtaining biomarker data.
7. The method according to claim 6 , wherein said biomarker probe is directed against at least one of the markers selected from the group consisting of p14Arf, p15INK4b, p16INK4a, p18INKc, p19INK4d, p21WAF1/CIP1, p27Kip1, Ki-67, Annexin A5, HPV viral proteins E1, E2, E3, E4, E5, E6, E7, L1, L2; and any combinations thereof.
8. The method according to claim 1 , wherein said obtained morphological data is compared to and correlated with a reference database.
9. The method according to claim 8 , wherein said obtained morphological data is stored in said reference database.
10. The method according to claim 6 , wherein said biomarker data is obtained by omitting background originating from labeled biomarker probe which is unbound.
11. The method according to claim 8 , wherein said reference database is a threshold database comprising a set of thresholds related to known cellular parameters in order to classify said cells with respect to their health status.