Cancer diagnostics using non-coding transcripts
Disclosed herein, in certain instances, are methods for the diagnosis, prognosis and determination of cancer progression of a cancer in a subject. Further disclosed herein, in certain instances, are methods for determining the treatment modality of a cancer in a subject. The methods comprise expression-based analysis of non-coding targets and coding targets. Further disclosed herein, in certain instances, are probe sets for use in assessing a cancer status in a subject.
1. A method of treating prostate cancer in a subject, comprising:
(a) obtaining or having obtained an expression level in a sample from a subject for a plurality of targets, wherein the plurality of targets comprises at least two targets selected from the group consisting of SEQ ID NOs: 103, 218, 275, 301, 321, 607, 707, 795, 828 and 832;
(b) determining that the subject is at risk of developing metastatic prostate cancer based on said expression level, or determining that the subject is not at risk of developing metastatic prostate cancer based on said expression level; and
(c) administering a prostate cancer treatment to the subject if the subject is determined to be at risk of developing metastatic prostate cancer based on said expression level, or monitoring without administering the prostate cancer treatment to the subject if the subject is determined to not be at risk of developing metastatic prostate cancer based on said expression level.
2. The method of claim 1 , wherein the treatment is selected from the group consisting of surgery, a chemotherapeutic agent, radiation therapy, a biological therapeutic, cancer vaccine, gene therapy or a combination thereof.
3. The method of claim 2 , wherein the treatment is prostatectomy, a chemotherapeutic agent, anti-androgen therapy or a combination thereof.
4. The method of claim 1 , wherein the sample is selected from the group consisting of tissue, blood, plasma, serum, urine, a urine supernatant, a urine cell pellet, semen, prostatic secretions and prostate cells.
5. The method of claim 1 , wherein said obtaining comprises a method selected from the group consisting of a sequencing technique, a nucleic acid hybridization technique, and nucleic acid amplification technique, and an immunoassay.
6. The method of claim 5 , wherein the nucleic acid amplification technique is polymerase chain reaction, reverse transcription polymerase chain reaction, transcription-mediated amplification, ligase chain reaction, strand displacement amplification or nucleic acid sequence-based amplification.
7. The method of claim 1 , wherein administering a prostate cancer treatment comprises administering cisplatin, carboplatin, oxaliplatin, mechlorethamine, cyclophosphamide, chlorambucil, ifosfamide, vincristine, vinblastine, vinorelbine, vindesine, paclitaxel, docetaxel, podophyllotoxin, irinotecan, topotecan, amsacrine, etoposide, etoposide phosphate, teniposide, actinomycin, anthracyclines, doxorubicin, daunorubicin, valrubicin, idarubicin, epirubicin, bleomycin, plicamycin, mitomycin, tositumomab, samarium-153-lexidronam, strontium-89 chloride, interferon alpha, interferon beta, interferon gamma, interleukin-2, interleukin 7, interleukin 12, G-CSF, GM-CSF, rituximab, trastuzumab, bacillus Calmette-Guerin (BCG), levamisole, porfimer sodium, mitoxantrone, prednisone, samarium, strontium, finasteride, or dutasteride.