Specimen concentration container and specimen concentrating method using same
A specimen concentration container contains a specimen-containing liquid mixture, and the liquid mixture is concentrated in the specimen concentration container. The specimen concentration container includes: a tubular container main body including an upper surface on which an upper surface opening portion is formed; an upper surface opening portion communicating with an inside of the container main body; a specimen concentration portion formed at a bottom portion side of the container main body and containing the concentrated liquid mixture; and a specimen lid provided in the container main body and configured to cover the upper surface opening portion.
1. A specimen concentration container comprising:
a container main body having an upper surface on which a first opening portion is formed;
a specimen concentration portion having a cup shape having an upper surface on which a second opening portion is formed and whose inner diameter is smaller than that of the container main body, the specimen concentration portion is provided at an inner bottom surface portion of the container main body; and
a specimen lid having a spherical shape whose diameter is larger than that of the second opening portion of the specimen concentration portion and is smaller than an inner diameter of the container main body, and the specimen lid is movably arranged on the second opening portion of the specimen concentration portion, the specimen lid is configured to float on a liquid mixture within the container and cover the second opening portion when the specimen concentration container is rotated under reduced pressure.
2. The specimen concentration container according to claim 1 , wherein the specimen lid is made of synthetic resin.
3. The specimen concentration container according to claim 1 , wherein the specimen lid is made of polypropylene resin.
4. The specimen concentration container according to claim 1 , wherein the specimen lid is made of polyethylene resin.
5. The specimen concentration container according to claim 1 , wherein the specimen lid is configured to float on the liquid mixture.
6. The specimen concentration container according to claim 5 , wherein the specimen lid includes therein a hollow portion.
7. The specimen concentration container according to claim 5 , wherein the specimen lid includes a columnar projecting portion projecting in one direction.
8. The specimen concentration container according to claim 7 , wherein an entire length of the specimen lid including the projecting portion is longer than an inner diameter of the container main body.
9. The specimen concentration container according to claim 1 , wherein a body lid is provided at the first opening portion of the container main body so as to open or close the first opening portion.
10. A specimen concentrating method using the specimen concentration container according to claim 1 , comprising:
arranging in the specimen concentration container the liquid mixture prepared by mixing the extraction liquid and a specimen;
evaporating a moisture in the liquid mixture;
rotating the specimen concentration container by a centrifuge under reduced pressure; and
sealing the specimen concentration portion by covering the second opening portion by a surface-contacting between the specimen lid and the container main body in the vicinity of the second opening portion to cover the second opening portion when an amount of the liquid mixture is reduced by evaporating and a liquid surface of the liquid mixture becomes lower than the second opening portion of the specimen concentration portion, wherein the specimen lid is configured to float on the liquid mixture.