Chromatography media and devices
Chromatography devices contain chromatography media and methods of making and methods of using chromatography devices. Chromatography devices enable a more efficient, productive and/or environmentally friendly chromatographic operation due to one or more of the following advantages over conventional chromatographic operations: elimination of a device packing step by the user; elimination of clean-in-place (CIP) steps; elimination of clean-in-place (CIP) steps utilizing sodium hydroxide solution; elimination of any validation steps by the user; and use of a chromatography device comprising biodegradable material. The chromatography media includes porous inorganic particles having a functionalized surface and having a median pore size of at least about 300 Angstroms (A), or at least about 300 A up to about 3000 A. The inorganic particles may have a BET surface area of at least about 20 m2/g, or at least about 25 m2/g, or about 30 m2/g, up to about 2000 m2/g.
1. A chromatography media comprising:
porous inorganic particles having a functionalized surface, said porous inorganic particles comprising silica particles having (i) a purity of at least 93% by weight SiO 2 based upon a total weight of the silica particles alone, (ii) a median particle dimension of from about 10 μm to about 120 μm, (iii) a median pore size of at least 300 Angstroms (Å) up to about 6000 Å, (iv) a pore volume of at least about 0.8 cc/g up to about 2.0 cc/g, (v) a pore size distribution relative span of at least about 0.8 up to about 2.0, and (vi) a BET surface area of from about 20 m 2 /g to about 2000 m 2 /g; and said functionalized surface comprises at least one molecule having a molecular weight of greater than about 300 g/mol up to about 500,000 g/mol,
wherein said functionalized surface comprises (i) polymer chains covalently bonded to and extending from a particle surface of the silica particles via a first set of functional groups of a first silane, the first set of functional groups enabling polymerization of one or more monomers onto the particle surface via the first silane, and (ii) a second set of functional groups covalently bonded to and extending from the particle surface via a second silane, the second set of functional groups increasing a wettability of the particle surface,
wherein said polymer chains are formed from (3-acrylamidopropyl) trimethylammonium chloride or 2-acrylamido-2-methyl-1-propanesulfonic acid.
2. The chromatography media of claim 1 , wherein said first silane comprises a vinyl silane and said second silane comprises an epoxy silane.