Droplet formation using fluid breakup
The present invention generally relates to systems and methods for creating droplets. In one aspect, a plurality of droplets ( 27 ) is introduced into a continuous fluid stream ( 21 ) to cause the continuous fluid stream to form discrete droplets. In some cases, the droplets that are formed from the continuous fluid stream may be substantially monodisperse. The continuous fluid stream may, in some cases, be a jetting fluid stream flowing at a relatively high linear flow rate, and in certain embodiments, high rates of droplet formation from the jetting fluid may thereby be achieved. Additionally, certain aspects of the invention are generally directed to devices, such as microfluidic devices, able to form such droplets. For example, in one set of embodiments, a device may include a junction ( 14 ) where a plurality of droplets ( 27 ) can be introduced into a continuous fluid stream ( 21 ), and optionally, the device may include additional junctions ( 12 ) able to cause the formation of the plurality of droplets and/or the formation of the continuous fluid stream. Still other disclosed aspects are generally directed to methods of making such devices, methods of using such devices, kits involving such devices, and the like.
1. A method of producing droplets, comprising:
providing a first continuous fluid stream comprising a first fluid, and a second continuous fluid stream comprising a second fluid and a plurality of insertable microfluidic droplets comprising a third fluid, the plurality of insertable droplets contained within the second fluid; and
inserting the plurality of insertable droplets into the first continuous fluid stream at a junction to cause the first continuous fluid stream to break up into discrete droplets of the first fluid, wherein the plurality of the insertable droplets and the discrete droplets exit the junction after the insertion of the plurality of the insertable droplets;
wherein the first continuous fluid stream comprising the first fluid is a fluid jet.
2. The method of claim 1 , wherein the first continuous fluid stream has a flow rate such that, in the absence of the insertion of the plurality of insertable droplets, the first continuous fluid stream does not form discrete droplets of first fluid.
3. The method of claim 1 , wherein the first continuous fluid stream has a Weber number (We) less than about 1.
4. The method of claim 1 , wherein the first continuous fluid stream has a Capillary number (Ca) greater than about 1.
5. The method of claim 1 , wherein the plurality of insertable droplets is inserted into the first continuous fluid stream at a rate of least about 15,000 droplets/s.
6. The method of claim 1 , wherein the plurality of insertable droplets are inserted into the first continuous fluid stream without substantially altering the linear flow rate of the continuous fluid stream.
7. The method of claim 1 , wherein the discrete droplets of the first fluid are substantially monodisperse.
8. The method of claim 1 , wherein the insertable droplets are substantially monodisperse.
9. The method of claim 1 , wherein the insertable droplets have a distribution in diameters such that no more than about 10% of the insertable droplets have a diameter that is less than about 90% of the overall average diameter of the insertable droplets.
10. The method of claim 1 , wherein the first continuous fluid stream further comprises an outer fluid surrounding at least a portion of the first fluid.
11. The method of claim 1 , wherein the first fluid, the second fluid, and the third fluid are each substantially mutually immiscible.
12. The method of claim 1 , wherein the first fluid and the second fluid are each liquids, and the third fluid is a gas.
13. The method of claim 1 , further comprising separating at least some of the third fluid from the first fluid after forming the discrete droplets of first fluid.
14. A method of producing droplets, comprising:
providing a first continuous fluid stream comprising a first fluid and a second continuous fluid stream comprising a second fluid, and a third fluid forming insertable microfluidic droplets within the second fluid; and
inserting a plurality of the insertable droplets of the third fluid into the first continuous fluid stream at a junction to cause the first continuous fluid stream to break up into discrete substantially monodisperse droplets of first fluid, wherein the plurality of the insertable droplets and the droplets of the first fluid exit the junction after insertion of the plurality of the insertable droplets;
wherein the first continuous fluid stream comprising the first fluid is a fluid jet.
15. The method of claim 14 , wherein the droplets of the first fluid have a distribution in diameters such that no more than about 10% of the droplets of the first fluid have a diameter that is less than about 90% of the overall average diameter of the droplets of the first fluid.
16. The method of claim 14 , wherein the third fluid is a gas.
17. A method of producing droplets, comprising:
providing a first continuous fluid stream comprising a first fluid;
providing a second continuous fluid stream comprising a second fluid containing substantially monodisperse droplets of a third fluid; and
inserting a plurality of substantially monodisperse droplets of the third fluid into the first continuous fluid stream at a junction to cause the first continuous fluid stream to form discrete droplets of the first fluid, wherein the droplets of the third fluid and the droplets of the first fluid exit the junction after insertion of droplets of the third fluid;
wherein the first continuous fluid stream comprising the first fluid is a fluid jet.
18. The method of claim 17 , wherein the third fluid is a gas.