System for hypothermic transport of samples
A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.
1. A method of preserving a biological sample, comprising:
introducing a biological sample to a transport container, wherein the transport container comprises:
a sample storage chamber configured to receive the biological sample;
a lid assembly comprising a fill port and a vent port, with at least a portion that is at an inclined angle with respect to a horizontal axis, and which seals against the sample storage chamber;
wherein the fill port provides a direct fluid path between the exterior of the transport container and the sample storage chamber; and
wherein the vent port provides a fluid path between a highest point of the lid assembly and the exterior of the transport container;
sealing the lid assembly to the sample storage chamber;
filling the sample storage chamber through the fill port with a preservation fluid; and
expelling excess preservation fluid from the transport container via the vent port.
2. The method of claim 1 , wherein filling comprises allowing the preservation fluid to exit the transport container from the vent port.
3. The method of claim 1 , wherein the preservation fluid comprises glucose, histidine, lactobionate, mannitol, raffinose, or sucrose.
4. The method of claim 1 , wherein the preservation fluid is selected from the group consisting of Collins, EuroCollins, phosphate buffered sucrose (PBS), University of Wisconsin (UW), histidine-tryptophan-ketoglutarate (HTK), hypertonic citrate, and hydroxyethyl starch.
5. The method of claim 1 ,
wherein the lid assembly further comprises:
a pumping chamber having a semi-permeable membrane disposed between the pumping chamber and the sample storage chamber at an inclined angle with respect to a horizontal axis and when the transport container is placed on a horizontal surface and a valve providing a fluidic path between a highest point of the sample storage chamber and the pumping chamber,
wherein the semi-permeable membrane pushes against the preservation fluid and causes the preservation fluid to circulate inside the transport container;
wherein the sample storage chamber is in fluidic communication with the pumping chamber; and
wherein the filling step further comprises filling the pumping chamber with the preservation fluid.
6. The method of claim 5 , wherein the semi-permeable membrane is inclined at an angle between approximately 1°-30° with respect to horizontal.
7. The method of claim 5 , wherein the valve is a ball check valve allows fluid flow from the sample storage chamber to the pumping chamber.
8. The method of claim 1 , wherein the transport container further comprises a source of oxygen-containing gas.
9. The method of claim 8 , wherein the source of oxygen-containing gas is in fluid communication with a first side of the semi-permeable membrane and provides a force against the semi-permeable membrane, thereby causing a second side of the semi-permeable membrane to push against the fluid and cause the fluid to circulate inside the transport container.
10. The method of claim 8 , wherein the source of oxygen-containing gas is a compressed gas cylinder.
11. The method of claim 1 , wherein the biological sample comprises tissues or organs.
12. The method of claim 1 , wherein the biological sample is a container holding body fluids.
13. The method of claim 1 , wherein the transport container further comprises an adapter coupling the biological sample to the pumping chamber.
14. The method of claim 1 , wherein the transport container further comprises a temperature sensor, a pressure sensor, or an oxygen sensor.