Devices for control of condensation and methods of use thereof
The present invention relates to devices including a heating element with an opening, which can control condensation of liquid samples during thermal incubation. The opening of the heating element can provide direct access to the samples in a process that may be automated. The invention also provides methods for heating containers.
1. A heating device, said device comprising:
(a) a frame comprising at least one well for holding a liquid sample,
(b) a heating element positioned above the frame, wherein the heating element does not contact the frame or the sample and is configured to heat the air above the sample, and
(c) an opening positioned within the heating element and above the at least one well, wherein the opening has a lateral cross-section sized to permit passage of a gripper or liquid dispenser to the well; wherein the heating element is configured to heat the opening to a temperature greater than the temperature of the well; and wherein the opening is configured to allow, throughout the device's use, continuous access to the well by the gripper or liquid dispenser.
2. The device of claim 1 , wherein the lateral cross-section of said opening is equal to, or larger than, the lateral cross-section area of said well.
3. The device of claim 1 , wherein said heating element heats said opening to a temperature 5, 10, 20, 30, 40, 50, 75, or 100 degrees centigrade greater than the temperature of said well.
4. The device of claim 1 , wherein said opening has a depth of between 5 millimeters (mm) and 200 mm.
5. The device of claim 2 , wherein said lateral cross-section of said opening is substantially rectangular.
6. The device of claim 5 , wherein said lateral cross-section has a first dimension of between 5 mm and 200 mm and has a second dimension of between 5 mm and 200 mm.
7. The device of claim 5 , wherein said depth is between 5 mm and 50 mm, and said lateral cross-section has a first dimension between 5 mm and 50 mm and a second dimension between 5 mm and 50 mm.
8. The device of any of claim 2 , wherein said lateral cross-section of said opening is circular or substantially oval.
9. The device of claim 1 , wherein said device comprises multiple wells.
10. The device of claim 9 , wherein said opening is positioned above said multiple wells.
11. The device of claim 1 , wherein said frame is a thermal cycler, vortexer, incubator, or agitator.
12. The device of claim 11 , wherein a second heating element is positioned below or about said frame.
13. The device of claim 12 , wherein one or both of said heating elements is a resistive heating element.
14. The device of claim 1 , wherein said heating element further comprising a robotic arm.
15. The device of claim 14 , wherein said heating element further comprising a gripper and a liquid dispenser.
16. The device of claim 15 , wherein said gripper and/or said liquid dispenser are automated.
17. A method of heating a liquid sample, said method comprising inserting said liquid sample into a well of the device of claim 1 , heating said liquid sample, and heating said opening.
18. A method of heating a liquid sample, said method comprising:
providing a liquid sample in a container, said container comprising a top surface, and
heating the top surface of said container, wherein the heat is provided by a heating element above said container, wherein the heating element does not contact the container and is configured to heat the air above the sample, wherein said heating element has an opening therewithin, wherein the opening has a lateral cross-section sized to permit passage of a gripper or liquid dispenser to the container, wherein the heating element heats the opening to a temperature greater than the temperature of the liquid sample, and wherein the opening is configured to allow, throughout the heating process, continuous access to the well by the gripper or liquid dispenser.
19. The method of claim 18 , said method further comprises providing a second heating element positioned below or about said container.
20. The method of claim 18 , wherein the top of said container contains a slit or a passage.
21. The method of claim 18 , further comprising providing a liquid dispenser.
22. The method of claim 21 , further comprising dispensing a liquid from said liquid dispenser through said top of said container while said container is positioned below said opening.
23. The method of claim 18 , further comprising:
providing a gripper;
passing said gripper through said opening;
gripping the top of said container with said gripper; and
passing said container through said opening using said gripper.
24. The method of claim 21 , further comprising providing a liquid dispenser and a gripper, wherein said liquid dispenser and said gripper are automated.
25. The method of claim 18 , wherein said heating element heats said opening to a temperature 10, 20, 30, 40, 50, 75, or 100 degrees centigrade greater than the temperature of said liquid sample.
26. A device for heating a liquid sample, said device comprising:
(a) a frame comprising at least one well for holding a liquid sample, wherein said frame is on an agitation device;
(b) a first heating element positioned above the frame, wherein the first heating element does not contact the frame or the sample and is configured to heat the air above the sample;
(c) an opening positioned within the heating element and above the at least one well, wherein the opening has a lateral cross-section sized to permit passage of a gripper or liquid dispenser to the well, wherein the opening is configured to allow, throughout the device's use, continuous access to the well by the gripper or liquid dispenser; and (d) a second heating element positioned below or about the frame; wherein the first heating element is configured to reach a higher temperature than the second heating element.