Compositions and methods to select for reduced grain moisture in maize
The invention relates to methods and compositions for identifying and selecting maize plants that have reduced grain moisture. Maize plants generated by the methods of the invention are also a feature of the invention.
1. A method of producing a maize plant with reduced grain moisture, the method comprising:
a. obtaining a sample of nucleic acids from maize germplasm;
b. detecting in said sample at least one marker allele located within
a chromosomal interval defined by and including PHM18206 and PHM4910, wherein the at least one marker allele is associated with a haplotype comprising a “T” at PHM9929-8, a “T” at PHM6667-11, a “C” at PHM229-15, a “T” at PHM6567-10, a “T” at PHM5208-11, a “C” at PHM15758-6, a “G” at PHM14370-9, a “C” at PHM3159-14, and a “T” at PHM17246-16;
c. selecting said maize germplasm that has the at least one marker allele that is associated with the haplotype comprising a “T” at PHM9929-8, a “T” at PHM6667-11, a “C” at PHM229-15, a “T” at PHM6567-10, a “T” at PHM5208-11, a “C” at PHM15758-6, a “G” at PHM14370-9, a “C” at PHM3159-14, and a “T” at PHM17246-16;
d. crossing said maize germplasm comprising said at least one marker allele with a second maize plant;
e. obtaining progeny seeds from said cross; and
f. growing at least one maize plant from said progeny seeds,
wherein said maize plant grown from said progeny seeds comprises said at least one marker allele and has reduced grain moisture when compared with a maize plant which does not comprise said at least one marker allele;
further wherein the maize plant is a Stiff Stalk Synthetic inbred or a progeny plant derived therefrom.
2. A method of producing a maize plant with reduced grain moisture, the method comprising:
a. obtaining a sample of nucleic acids from maize germplasm;
b. detecting in said sample a haplotype comprising a “T” at PHM9929-8, a “T” at PHM6667-11, a “C” at PHM229-15, a “T” at PHM6567-10, a “T” at PHM5208-11, a “C” at PHM15758-6, a “G” at PHM14370-9, a “C” at PHM3159-14, and a “T” at PHM17246-16;
c. selecting said maize germplasm that has the haplotype;
d. crossing said maize germplasm from (c) with a second maize plant;
e. obtaining progeny seeds from said cross; and
f. growing at least one maize plant from said progeny seeds,
wherein said maize plant grown from said progeny seeds comprises the haplotype and has reduced grain moisture when compared with a maize plant which does not comprise the haplotype;
further wherein the maize plant is a Stiff Stalk Synthetic inbred or a progeny plant derived therefrom.