Biosynthetic gene cluster for chejuenolide of marine microorganism
The present invention relates to a biosynthetic gene cluster for a chejuenolide of the marine microorganism Hahella chejuensis , and to the function of an enzyme involving the biosynthetic pathway of a chejuenolide encoded by the genes. Since the present invention can be applied to the development of a novel material as a mechanism for biosynthesis by combining of biosynthetic genes for chejuenolide, the present invention can suggest a new direction in the study of polyketide antibiotics, and is thus very useful.
1. A transformant microorganism transformed from a microorganism having a genetic sequence of SEQ ID NO: 1 in its genome, wherein one or more genes encoding amino acid sequences of SEQ ID NOS: 2 to 27 that are included the SEQ ID: 1 are disrupted in the transformant microorganism.
2. The transformant microorganism according to claim 1 , wherein the microorganism is a mutant strain Hahella chejuensis O3KO (International Accession No.: KCTC 12315BP) in which a gene encoding an amine oxidase in the DNA sequence of SEQ ID NO: 1 is disrupted.
3. A method of preparing a precursor of chejuenolide, comprising:
(1) culturing a mutant strain in which one or more of genes encoding proteins having amino acid sequences set forth in SEQ ID NOS: 2 to 27 are disrupted by knockout system, wherein the mutant strain includes a chejuenolide biosynthetic gene set forth in SEQ ID NO: 1 in the genome thereof; and
(2) obtaining the precursor of chejuenolide from the culture broth.
4. The method according to claim 3 , wherein the mutant strain is disrupted at a 20,900th to 19,482nd region (orf3) encoding an amine oxidase having an amino acid sequence set forth in SEQ ID NO: 9 by knockout system.
5. The method according to claim 4 , wherein the mutant strain is a Hahella chejuensis O3KO (International Accession No.: KCTC 12315BP).
6. The method according to claim 3 , wherein the precursor is a chejuenolide precursor O3P2 represented by Formula 1.