Method for preserving enzyme
The problem is to provide a method for inexpensively and simply preserving an enzyme of a microbial biomass obtained by culturing and improve the enzyme activity during preservation. Provided is a method for preserving nitrile hydratase, characterized in that microbes having nitrile hydratase activity are cultured while being protected from light, and the resulting biomass is crushed using a high-pressure homogenizer and preserved while being protected from light.
1. A method for preserving nitrile hydratase, said method comprising:
(1) culturing a microorganism having a nitrile hydratase activity while protecting them from light,
(2) disrupting the cultured microbial cells from the microorganism using a high-pressure type homogenizer, and
(3) preserving the disrupted microbial cells for 4 to 24 hours at 4 to 37° C. while protecting them from light.
2. The method according to claim 1 , wherein the microorganism having a nitrile hydratase activity is a microorganism belonging to the genus Rhodococcus or the genus Pseudonocardia.
3. The method of claim 1 , wherein the microorganism having a nitrile hydratase activity belongs to the genus Rhodococcus, Pseudonocardia, Pseudomonas, Pseudonocardia , or Geobacillus , or is a genetic recombinant microorganism with an introduced nitrile hydratase gene.
4. A method for activating nitrile hydratase, said method comprising:
(1) culturing a microorganism having a nitrile hydratase activity while protecting them from light,
(2) disrupting the cultured microbial cells from the microorganism using a high-pressure type homogenizer, and
(3) preserving the disrupted microbial cells for 4 to 24 hours at 4 to 37° C. while protecting them from light.
5. The method according to claim 4 , wherein the microorganism having a nitrile hydratase activity is a microorganism belonging to the genus Rhodococcus or the genus Pseudonocardia.
6. The method of claim 4 , wherein the microorganism having a nitrile hydratase activity belongs to the genus Rhodococcus, Pseudonocardia, Pseudomonas, Pseudonocardia , or Geobacillus , or is a genetic recombinant microorganism with an introduced nitrile hydratase gene.
7. A method for producing activated nitrile hydratase, said method comprising:
(1) culturing a microorganism having a nitrile hydratase activity while protecting them from light,
(2) disrupting the cultured microbial cells from the microorganism using a high-pressure type homogenizer, and
(3) preserving the disrupted microbial cells for 4 to 24 hours at 4 to 37° C. while protecting them from light.
8. The method according to claim 7 , wherein the microorganism having a nitrile hydratase activity is a microorganism belonging to the genus Rhodococcus or the genus Pseudonocardia.
9. The method of claim 7 , wherein the microorganism having a nitrile hydratase activity belong to the genus Rhodococcus, Pseudonocardia, Pseudomonas, Pseudonocardia , or Geobacillus , or is a genetic recombinant microorganism with an introduced nitrile hydratase gene.
10. A method for producing nitrile hydratase, said method comprising:
(1) culturing a microorganism having a nitrile hydratase activity while protecting them from light,
(2) disrupting the cultured microbial cells from the microorganism using a high-pressure type homogenizer, and
(3) preserving the disrupted microbial cells while protecting them from light.
11. The method according to claim 10 , wherein the microorganism having a nitrile hydratase activity is a microorganism belonging to the genus Rhodococcus or the genus Pseudonocardia.
12. The method according to claim 10 , wherein the preservation of the disrupted microbial cells is performed at a temperature of from 4 to 37° C.
13. The method of claim 10 , wherein the microorganism having a nitrile hydratase activity belong to the genus Rhodococcus, Pseudonocardia, Pseudomonas, Pseudonocardia , or Geobacillus , or is a genetic recombinant microorganism with an introduced nitrile hydratase gene.