ANTIBODY PURIFICATION METHOD, AND CARRIER FOR USE IN PURIFICATION OF ANTIBODY
An antibody purification method includes: bringing a solution that includes an antibody and has a pH of 3.0 or more and less than 5.6 into contact with a carrier that includes a 2,3-dihydroxypropyl group to adsorb the antibody on the carrier; and bringing the carrier that has been brought into contact with the solution, into contact with an eluent having a pH of 5.6 or more and less than 10 to elute the antibody from the carrier.
1 . An antibody purification method comprising:
contacting a solution comprising an antibody and having a pH of 3.0 or more and less than 5.6 with a carrier comprising a 2,3-dihydroxypropyl group; and then
contacting the carrier with an eluent having a pH of 5.6 or more and less than 10.
2 . An antibody purification carrier comprising a 2,3-dihydroxypropyl group, wherein the 2,3-dihydroxypropyl group is present on a surface of the antibody purification carrier.
3 . The antibody purification carrier according to claim 2 , wherein the antibody purification carrier comprises a porous particle.
4 . The antibody purification carrier according to claim 2 , wherein the antibody purification carrier comprises a particle comprising a magnetic material.
5 . The antibody purification carrier according to claim 3 , wherein the antibody purification carrier comprises a particle comprising a magnetic material.
6 . The antibody purification method according to claim 1 , wherein the solution has a pH of 3.5 or more and less than 5.6.
7 . The antibody purification method according to claim 1 , wherein the eluent has a pH of 6.0 or more and less than 10.
8 . The antibody purification method according to claim 1 , wherein the solution has a pH of 3.5 or more and less than 5.6, and the eluent has a pH of 6.0 or more and less than 10.