Nucleotide sequences encoding fasciated EAR3 (FEA3) and methods of use thereof
Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
1. A method of making a maize plant in which expression of an endogenous FEA3 gene, which encodes a polypeptide having an amino acid sequence of at least 95% sequence identity, based on the Clustal W method of alignment, when compared to SEQ ID NO:3 or 5, is reduced relative to a control plant, the method comprising the steps of:
a. introducing a mutation into the endogenous FEA3 gene; and
b. detecting the mutation
and wherein the maize plant comprising the mutant FEA3 gene exhibits ears that show a progressive enlargement of the tip, extra kernel rows or an overall irregular arrangement of rows as compared to a wild type maize plant.
2. The method of claim 1 wherein using the steps (a) and (b) are done using a Targeting Induced Local Lesions IN Genomics (TILLING) method and wherein the mutation is effective in reducing the expression of the endogenous FEA3 gene or its activity.
3. The method of claim 1 wherein the mutation is a site-specific mutation.
4. The method of claim 1 , wherein the mutation comprises a transposon inserted into the FEA3 gene sequence.