IP Library Granted Patent US 9,580,428
Granted Patent B2
US 9,580,428 · App. 14/395,355 · Granted Feb 28, 2017

Compositions and methods for inhibiting viral polymerase

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Quick Facts
Patent No.
US 9,580,428
App. No.
14/395,355
Granted
Feb 28, 2017
Kind
B2
Abstract

Provided are compounds of Formula I: as described herein. Compounds of Formula I are useful in methods of inhibiting viral RNA polymerase activity and viral replication. Also provided are pharmaceutical compositions comprising compounds of Formula I, as well as methods of treating viral infections using compounds of Formula I.

Claims (46)

1. A compound represented by Formula (I), or a pharmaceutically acceptable salt thereof:

wherein:

L 1 , L 2 , and L 3 , each independently, are a bond or —C(R 0 ) 2 —O—;

wherein when L 1 , L 2 , or L 3 is —C(R 0 ) 2 —O—, the —C(R 0 ) 2 — moiety of the —C(R 0 ) 2 —O— group is attached to the oxygen on the pyrrolidine ring and the —O— atom of the —C(R 0 ) 2 —O— group is attached to R 1 , R 2 , or R 3 , respectively;

L 4 , L 5 , and L 6 are each a bond;

R 0 , independently for each occurrence, is H or (C 1 -C 6 )alkyl;

R 1 , R 2 , and R 3 , each independently, are selected from the group consisting of H, aminoacyl, aminothionyl, acyl, R 10 OC(O)—, phosphoryl, and aminophosphoryl;

or R 1 and R 2 , taken together, or R 2 and R 3 , taken together, may be selected from the group consisting of carbonyl, thiocarbonyl, phosphoryl, and (C 1 -C 6 )alkylphosphoryl;

R 4 , R 5 , and R 6 are each H;

R 7 is H;

R 10 , independently for each occurrence, is selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, heteroaryl, aralkyl, and heteroaralkyl;

provided that the compound represented by Formula (I) is not

2. The compound of claim 1 , wherein L 1 -R 1 and L 2 -R 2 are identical, L 2 -R 2 and L 3 -R 3 are identical, or L 1 -R 1 and L 3 -R 3 are identical.

3. The compound of claim 2 , wherein L 3 -R 3 is H.

4. The compound of claim 2 , wherein L 1 -R 1 is H.

5. The compound of claim 2 , wherein L 2 -R 2 is H.

6. The compound of claim 1 , wherein L 1 -R 1 , L 2 -R 2 , and L 3 -R 3 are identical.

7. The compound of claim 1 , wherein independently for each occurrence aminoacyl is —C(═O)CH(NH 2 )(CH 2 ) n CHR 30 R 31 , wherein

n is 0 or 1; and

R 30 and R 31 each independently are selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, heteroaryl, aralkyl, and heteroaralkyl.

8. The compound of claim 1 , wherein independently for each occurrence aminothionyl is —C(═S)CH(NH 2 )(CH 2 ) n CHR 30 R 31 , wherein

n is 0 or 1; and

R 30 and R 31 each independently are selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, heteroaryl, aralkyl, and heteroaralkyl.

9. The compound of claim 1 , wherein independently for each occurrence acyl is —C(═O)R 40 , wherein R 40 is selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, heteroaryl, aralkyl, and heteroaralkyl.

10. The compound of claim 1 , wherein independently for each occurrence aminophosphoryl is —P(═O)(OR 50 )NR 51 R 52 , wherein

R 50 is selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, arylalkyl, heteroaryl, heteroaralkyl, and -(CH 2 ) m SC(═O)C(CH 3 ) 2 CH 2 OH;

m is 1 or 2;

R 51 is H or (C 1 -C 6 )alkyl; and

R 52 is selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, and —CR 60 R 61 C(═O)OR 62 , wherein

R 60 and R 61 each independently are H or (C 1 -C 6 )alkyl; and

R 62 is selected from the group consisting of H, (C 1 -C 6 )alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl.

11. The compound of claim 10 , wherein R 52 is —CR 60 R 61 C(═O)OR 62 .

12. The compound of claim 11 , wherein R 60 is H; R 61 is (C 1 -C 6 )alkyl; and R 62 is (C 1 -C 6 )alkyl.

13. The compound of claim 1 , selected from the group consisting of:

and pharmaceutically acceptable salts thereof.

14. The compound of claim 1 represented by

or a pharmaceutically acceptable salt thereof.

15. The compound of claim 1 , selected from the group consisting of:

(S)-((2R,3R,4S,5S)-5-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-3,4-dihydroxypyrrolidin-2-yl)methyl 2-amino-3-methylbutanoate;

(2S,3S)-((2R,3R,4S,5S)-5-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-3,4-dihydroxypyrrolidin-2-yl)methyl 2-amino-3-methylpentanoate;

(S)-((2R,3R,4S,5S)-5-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-3,4-dihydroxypyrrolidin-2-yl)methyl 2-amino-4-methylpentanoate;

(2S,2′S)-(2S,3S,4R,5R)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-5-(hydroxymethyl)pyrrolidine-3,4-diyl bis(2-amino-3-methylbutanoate);

(S)-(2R,3R,4S,5S)-5-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-3-yl 2-amino-3-methylbutanoate;

(S)-(2S,3S,4R,5R)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-4-hydroxy-5-(hydroxymethyl)pyrrolidin-3-yl 2-amino-3-methylbutanoate; and

pharmaceutically acceptable salts thereof.

16. A pharmaceutical composition, comprising a compound of claim 1 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

Assignments (6)
RELEASE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Apr 18, 2023
From: ATHYRIUM OPPORTUNITIES III CO-INVEST 1 LP
To: BIOCRYST PHARMACEUTICALS, INC.
Reel/Frame 063362/0550 →
RELEASE OF SECURITY INTEREST Recorded Apr 6, 2023
From: MIDCAP FINANCIAL TRUST
To: BIOCRYST PHARMACEUTICALS, INC.; MDCP, LLC
Reel/Frame 063348/0762 →
SECURITY INTEREST Recorded Dec 17, 2020
From: BIOCRYST PHARMACEUTICALS, INC.
To: ATHYRIUM OPPORTUNITIES III CO-INVEST 1 LP
Reel/Frame 054800/0634 →
RELEASE OF SECURITY INTEREST Recorded Dec 16, 2020
From: MIDCAP FINANCIAL TRUST, AS AGENT
To: BIOCRYST PHARMACEUTICALS, INC.; MDCP, LLC
Reel/Frame 054774/0446 →
SECURITY INTEREST Recorded Sep 26, 2016
From: BIOCRYST PHARMACEUTICALS, INC.; MDCP, LLC
To: MIDCAP FINANCIAL TRUST, AS AGENT
Reel/Frame 039858/0967 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 16, 2015
From: KOTIAN, PRAVIN L.; BABU, YARLAGADDA S.
To: BIOCRYST PHARMACEUTICALS, INC.
Reel/Frame 035427/0667 →