IP Library Granted Patent US 10,047,342
Granted Patent B2
US 10,047,342 · App. 14/399,406 · Granted Aug 14, 2018

Elutriation chamber for an elutriator system

Inventors: Johann Eibl (Vienna, AT); Johann Graus (Vienna, AT); Christoph Mader (Korneuburg, AT)
Assignee: BIO-PRODUCTS & BIO-ENGINEERING AG
C12N5/0644A61M1/3692A61M1/3693A61M1/3696B01D21/262B04B5/0442C12M47/04A61M2202/0427B01D2221/10B04B2005/0471
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Quick Facts
Patent No.
US 10,047,342
App. No.
14/399,406
Granted
Aug 14, 2018
Kind
B2
Abstract

The invention relates to an elutriation chamber for an elutriator system for washing and/or isolating cells, in particular thrombocytes, which elutriation chamber comprises a feed line ( 1 ) for an aqueous medium containing the cells to be washed and/or to be isolated in suspended form, and a discharge line ( 2 ) for the washed and/or isolated cells, wherein the chamber ( 5 ) is rotationally symmetrical to the axis (a), characterized in that the ratio of the area of the section through the lumen of the chamber ( 5 ) perpendicular to the axis (a) at the widest point ( 5 a - 5 b ) to the area of the section ( 1 a ) through the feed line ( 1 ) is in the range of 1,000 to 250,000.

Claims (21)

1. An elutriation device for an elutriator system, the elutriation device having a chamber for washing and/or isolating cells, in particular thrombocytes, which elutriation device comprises:

a feed line for an aqueous medium containing the cells to be washed and/or to be isolated in suspended form,

a chamber lumen,

a discharge line for the washed and/or isolated cells, and

an axis extending from the feed line to the discharge line,

wherein a ratio of an area of a section through the chamber lumen perpendicular to the axis at a widest point to the area of a section through the feed line is in a range of 1,000 to 250,000.

2. An elutriation device according to claim 1 , further comprising a further feed line for a gaseous or liquid medium.

3. An elutriation device for an elutriator system, the elutriation device having a chamber for washing and/or isolating cells, in particular thrombocytes, which elutriation device comprises:

a chamber entrance,

a feed line coupled to the chamber at the chamber entrance, the feed line having a circular cross-section for passing an aqueous medium containing the cells to be washed and/or to be isolated in suspended form through the narrowing of the chamber entrance and into the chamber,

a lumen of the chamber,

a discharge line for the washed and/or isolated cells, and

an axis extending from the feed line to the discharge line,

wherein, in the feed line at the chamber entrance, a narrowing is provided in such a way that a ratio between an area of a section through the lumen of the chamber perpendicular to the axis at a widest point to an area of a section through the narrowing is in a range of 1,000 to 250,000.

4. An elutriation device according to claim 1 , further comprising a chamber entrance provided with a narrowing, the feed line being coupled to the chamber at the chamber entrance such that the aqueous medium containing the cells is passed through the narrowing of the chamber entrance and into the chamber.

5. An elutriation device according to claim 3 , further comprising a further feed line for a gaseous or liquid medium.

6. An elutriation device according to claim 1 , wherein the chamber is rotationally symmetrical about the axis.

7. An elutriation device according to claim 1 , wherein the feed line has a circular cross-section.

8. An elutriation device according to claim 3 , wherein the narrowing at the chamber entrance is conical.

9. An elutriation device according to claim 4 , wherein the narrowing at the chamber entrance is conical.

10. An elutriation device according to claim 3 , wherein the chamber is rotationally symmetrical about the axis.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 25, 2014
From: EIBL, JOHANN; GRAUS, JOHANN; MADER, CHRISTOPH
To: BIO-PRODUCTS & BIO-ENGINEERING AG
Reel/Frame 034259/0653 →
Priority Claims (1)
AT A 50173/2012 · May 11, 2012 · national
Continuity (1)
Related Publication 20150111295A1 · Apr 23, 2015
Cited By (14)
US 1,109,621 US 12,305,156 US 12,337,321 US 12,350,664 US 12,350,667 US 12,350,668 US 12,399,193 US 12,403,468 US 12,491,512 US 12,492,368 US 12,497,587 US 12,551,887 US 12,559,710 US 12,570,948