METHOD FOR STERILIZING MEMBRANE COMPRISING GLUCOSE OXIDASE AND ASSOCIATED BIO-SENSOR
A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprises: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution. Associated biosensors and bioreactors are also described.
1 . A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprising: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution.
2 . The method of claim 1 , wherein the oxidoreductase enzyme is selected from the group consisting of glucose oxidase, lactate oxidase and glutamate oxidase.
3 . The method of claim 2 , wherein the oxidoreductase enzyme is glucose oxidase.
4 . The method of claim 1 , wherein the aqueous buffer solution is a phosphate buffer.
5 . The method of claim 4 , wherein the aqueous buffer solution is a phosphate buffer saline (PBS) solution.
6 . The method of claim 1 , wherein the aqueous buffer solution is substantially free from organic components.
7 . A method for sterilizing a membrane comprising glucose oxidase, comprising: irradiating with gamma radiation the membrane comprising glucose oxidase soaked in a phosphate buffer saline (PBS) solution.
8 . The method of claim 1 , wherein a dose of the gamma radiation is in a range of from about 25 kGy to about 40 kGy.
9 . The method of claim 1 , wherein a rate of the gamma radiation is about 20 kGy/hour.
10 . The method of claim 1 , wherein the membrane is in a plastic container.
11 . The method of claim 1 , wherein the membrane is in a biosensor.
12 . The method of claim 11 , wherein the biosensor comprises a Pt electrode and an Ag/AgCl electrode.
13 . A biosensor comprising the membrane sterilized using the method of claim 1 .
14 . The biosensor of claim 13 , wherein the membrane comprises glucose oxidase and the sensitivity of the biosensor to glucose is about 80% of the sensitivity of a biosensor comprising a membrane comprising glucose oxidase without sterilization to glucose.
15 . The biosensor of claim 13 , comprising a Pt electrode and an Ag/AgCl electrode.
16 . A biosensor comprising a membrane comprising glucose oxidase, sterilized by: irradiating with gamma radiation while the membrane comprising glucose oxidase is soaked in a phosphate buffer saline (PBS) solution.
17 . A bioreactor comprising the biosensor of claim 13 .