Compositions and methods for biophotonic bone reconstruction
This disclosure provides biophotonic compositions comprising a photoactivator, a calcium phosphate mineral, hyaluronic acid and optionally glucosamine. The compositions of this disclosure have utility in the augmentation, repair and/or regeneration of bone when used in conjunction with actinic light of a wavelength absorbed by the photoactivator.
1. A biophotonic composition comprising:
a photoactivator which can absorb and emit light;
a calcium phosphate mineral having an average particle size of between about 150 nm and about 500 nm;
hyaluronic acid; and
glucosamine;
wherein the hyaluronic acid and the glucosamine components are about 70-90% by weight of the total weight of the composition;
wherein the biophotonic composition is non-photo-polymerized and is suitable for administration at a site of bone reconstruction;
wherein the biophotonic composition emits fluorescence upon being illuminated by actinic light for a period of between about 1 second and 30 seconds; and
wherein the biophotonic composition does not include an oxidant.
2. The biophotonic composition of claim 1 , wherein the photoactivator is present in an amount of at least about 0.2% by weight of the total weight of the biophotonic composition.
3. The biophotonic composition claim 1 , wherein the photoactivator is present in an amount of about 0.2-1% by weight of the total weight of the biophotonic composition.
4. The biophotonic composition of claim 1 , wherein the photoactivator is a xanthene dye or a fluorescein derivative.
5. The biophotonic composition of claim 4 , wherein the fluorescein derivative is eosin Y.
6. The biophotonic composition of claim 1 , wherein the calcium phosphate mineral comprises hydroxyapatite.
7. The biophotonic composition of claim 1 , wherein the calcium phosphate mineral is about 10-30% by weight of the total weight of the biophotonic composition.
8. The biophotonic composition of claim 1 , wherein the hyaluronic acid is cross-linked hyaluronic acid.
9. The biophotonic composition of claim 1 , wherein the hyaluronic acid is a non cross-linked hyaluronic acid.
10. The biophotonic composition of claim 9 , wherein the hyaluronic acid has a molecular weight of between about 1 million Dalton and 2 million Dalton, about 1.2 million to about 1.8 million Dalton, or about 1.7 million Dalton.
11. The biophotonic composition of claim 1 , wherein the hyaluronic acid is about 5-90% by weight of the total weight of the biophotonic composition.
12. The biophotonic composition of claim 1 , wherein the ratio of calcium phosphate mineral to hyaluronic acid is about 1:9, 1.5:8.5, 2:8, 2.5:7.5 or 3:7.
13. The biophotonic composition of claim 1 , wherein the ratio of calcium phosphate mineral to hyaluronic acid is about 6:4, 6.5:3.5 or 7:3.
14. The biophotonic composition of claim 1 , wherein the ratio of calcium phosphate mineral to hyaluronic acid is about 8:2, 8.5:1.5, 9:1 or 9.5:0.5.
15. The biophotonic composition of claim 1 , wherein the ratio of hyaluronic acid to glucosamine is about 1:1, 3:2, 7:3, 4:1; or 9:1.
16. The biophotonic composition of claim 1 , wherein the ratio of calcium phosphate mineral to hyaluronic acid and glucosamine is about 1:9, 1.5:8.5, 2:8, 2.5:7.5 or 3:7.
17. The biophotonic composition of claim 1 , wherein the ratio of calcium phosphate mineral to hyaluronic acid and glucosamine is about 6:4, 6.5:3.5 or 7:3.
18. The biophotonic composition of claim 1 , wherein the ratio of calcium phosphate mineral to hyaluronic acid and glucosamine is about 8:2, 8.5:1.5, 9:1 or 9.5:0.5.
19. The biophotonic composition of claim 1 , wherein the biophotonic composition does not include one or more of triethanolamine (TEA), N-vinyl-2-pyrrolidone (NVP), or N-vinyl caprolactam (NVC).
20. The biophotonic composition of claim 1 , wherein the biophotonic composition does not include a 15 amino acid residue peptide irreversibly bound to the calcium phosphate mineral.
21. The biophotonic composition of claim 20 , wherein the calcium phosphate mineral comprises hydroxyapatite.
22. A method for augmenting, repairing or regenerating bone, comprising:
a) providing a biophotonic composition of claim 1 ;
b) applying a layer of the biophotonic composition to a bone tissue site;
c) irradiating the biophotonic composition with actinic light; and d) repeating steps (b) and (c) at least once.
23. The method of claim 22 , wherein each layer of step (b) has a thickness of about 0.5-4 mm.
24. The method of claim 22 , wherein the biophotonic composition is irradiated for less than about 5 minutes.
25. The method of claim 22 , wherein the biophotonic composition is irradiated with light having a wavelength in the range of about 400-700 nm.
26. The method of claim 22 , wherein the biophotonic composition promotes detectable bone growth in the bone tissue site in about 3 months.
27. The method of claim 22 , wherein the biophotonic composition used for a first layer has a different ratio of calcium phosphate mineral to hyaluronic acid and/or glucosamine than the biophotonic composition used for a second layer.