Pulmonary delivery of mRNA to non-lung target cells
View Patent ↗Compositions comprising mRNA formulated for pulmonary administration and related methods for delivery of the mRNA and/or encoded protein to a non-lung cell or tissue. The compositions and methods may be used to prevent or ameliorate the symptoms of diseases associated with the mRNA encoded protein.
1. A method of delivery of messenger RNA (mRNA) to a non-lung cell or tissue comprising administering to the lung by nebulization a composition comprising mRNA encoding a protein and a lipid carrier vehicle comprising one or more PEG-modified lipids, wherein the administering to the lung results in the delivery of the mRNA to a non-lung cell or tissue, and wherein the protein encoded by the mRNA is detectable in the non-lung cell or tissue for at least 6 hours following the administration to the lung.
2. The method of claim 1 , wherein the non-lung cell is selected from the group consisting of hepatocytes, epithelial cells, hematopoietic cells, epithelial cells, endothelial cells, bone cells, stem cells, mesenchymal cells, neural cells, cardiac cells, adipocytes, vascular smooth muscle cells, cardiomyocytes, skeletal muscle cells, beta cells, pituitary cells, synovial lining cells, ovarian cells, testicular cells, fibroblasts, B cells, T cells, reticulocytes, leukocytes, granulocytes, macrophages, neutrophils, antigen presenting cells (dendritic cells), fibroblasts, tumor cells and combination thereof.
3. The method of claim 1 , wherein the non-lung tissue is selected from the group consisting of heart, liver, spleen, kidneys, skeletal muscle, lymph nodes, skin, brain, cerebrospinal fluid, plasma and combination thereof.
4. The method of claim 1 , wherein the mRNA encodes a protein selected from the group consisting of a cytosolic protein, a secreted protein and a therapeutic protein.
5. The method of claim 4 , wherein the cytosolic protein is selected from the group consisting of enzymes, transcription factors, chaperones, and combination thereof.
6. The method of claim 4 , wherein the therapeutic protein is selected from the group consisting of alpha galactosidase, erythropoietin, a1-antitrypsin, carboxypeptidase N, alpha-L-iduronidase, iduronate-2-sulfatase, N-acetylglucosamine-1-phosphate transferase, N-acetylglucosaminidase, lysosomal acid lipase, arylsulfatase-A alpha-glucosaminide acetyltransferase, N-acetylglucosamine 6-sulfatase, N-acetylgalactosamine-4-sulfatase, beta-glucosidase, galactose-6-sulfate sulfatase, beta-galactosidase, beta-glucuronidase, glucocerebrosidase, heparan sulfamidase, hyaluronidase, galactocerebrosidase, human growth hormone, ornithine transcarbamylase (OTC), carbamyl phosphate synthetase-1 (CPS1), argininosuccinate synthetase-1 (ASS1), argininosuccinate lyase (ASL), arginase-1 (ARG1), cystic fibrosis transmembrane conductance regulator (CFTR), Factor VII, Factor VIII, Factor IX, heparan-N-sulfatase, and combination thereof.
7. The method of claim 1 , wherein the protein is detectable in the non-lung cell or tissue for at least about 1 day or for at least about 2 days following the administration to the lung.
8. The method of claim 1 , wherein the mRNA is delivered at an amount greater than about 0.5 mg/kg of mRNA per dose.
9. The method of claim 1 , wherein the lipid carrier vehicle is a liposome.
10. The method of claim 9 , wherein the liposome further comprises one or more cationic lipids, one or more non-cationic lipids, and one or more cholesterol-based lipids.
11. The method of claim 10 , wherein the one or more cationic lipids are selected from the group consisting of C12-200, HGT4003, HGT5000, HGT5001, ICE, DLinKC2-DMA, DODAP, DODMA, DLinDMA, CLinDMA, and combination thereof.
12. The method of claim 1 , wherein the composition further comprises one or more pulmonary surfactants.
13. The method of claim 1 , wherein the composition is formulated as respirable particles.
14. The method of claim 13 , wherein the respirable particles have a size less than about 500 μm.
15. The method of claim 1 , wherein the composition is formulated as nebulizable lipid or as dry powder.
16. The method of claim 1 , wherein the non-lung cell or tissue is affected by a disease or disorder.
17. The method of claim 16 , wherein the disease or disorder is selected from Table 4.
18. The method of claim 1 , wherein the mRNA is unmodified.