Antibody specific for CLEC14A and uses thereof
Provided is an antibody specifically binding to the CTLD (C-type lectin like domain) of clec14a (C-type lectin domain family 14, member A), a method for preparing the antibody, a composition for suppressing angiogenesis comprising the antibody, a method for suppressing angiogenesis by administering the antibody or the composition, a composition for preventing or treating cancer comprising the antibody, a method for treating cancer by administering the antibody or the composition, a composition for diagnosing cancer comprising the antibody, a kit for diagnosing cancer comprising the composition, a method for diagnosing cancer using the composition, a composition for suppressing angiogenesis comprising a material for inhibiting expression of clec14a, a kit for angiogenesis comprising the composition, a method for suppressing angiogenesis or treating cancer using the composition, and the use of the CTLD of clec14a as an epitope for an antibody suppressive of angiogenesis.
1. An antibody, binding specifically to CTLD (C-type lectin like domain) of clec14a (C-type lectin domain family 14, member A), wherein the antibody comprises at least one selected from the group consisting of:
(a) a heavy-chain variable region comprising heavy-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 14, heavy-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 16, and heavy-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 18, and a light-chain variable region comprising light-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 42, light-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 44, and light-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 46;
(b) a heavy-chain variable region comprising heavy-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 21, heavy-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 23, and heavy-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 25, and a light-chain variable region comprising light-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 49, light-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 51, and light-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 53;
(c) a heavy-chain variable region comprising heavy-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 28, heavy-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 30, and heavy-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 32, and a light-chain variable region comprising light-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 56, light-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 58, and light-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 60; and
(d) a heavy-chain variable region comprising heavy-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 35, heavy-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 37, and heavy-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 39, and a light-chain variable region comprising light-chain CDR1 defined by an amino acid sequence of SEQ ID NO: 63, light-chain CDR2 defined by an amino acid sequence of SEQ ID NO: 65, and light-chain CDR3 defined by an amino acid sequence of SEQ ID NO: 67.
2. The antibody of claim 1 , wherein the antibody is selected from the group consisting of:
an antibody comprising a heavy-chain variable region having an amino acid sequence of SEQ ID NO: 125 and a light-chain variable region having an amino acid sequence of SEQ ID NO: 129;
an antibody comprising a heavy-chain variable region having an amino acid sequence of SEQ ID NO: 126 and a light-chain variable region having an amino acid sequence of SEQ ID NO: 130;
an antibody comprising a heavy-chain variable region having an amino acid sequence of SEQ ID NO: 127 and a light-chain variable region having an amino acid sequence of SEQ ID NO: 131; and
an antibody comprising a heavy-chain variable region having an amino acid sequence of SEQ ID NO: 128 and a light-chain variable region having an amino acid sequence of SEQ ID NO: 132.
3. An antibody-drug conjugate comprising the antibody of claim 1 attached to a drug, wherein the drug is any one selected from the group consisting of a toxin, a chemotherapeutic agent, an anticancer drug, an antibiotic, ADP-ribosyl transferase, a radioactive isotope and a nucleolytic enzyme.
4. A diagnostic kit for angiogenesis-related disease, comprising the antibody of claim 1 .