Carbohydrate detection
The present invention relates to a method for detection, identification and/or quantification of one or more carbohydrates. The method comprises the steps of contacting an objector a sample with a luminescent conjugated oligothiophene (LCO) and detecting at least one detection signal of the luminescent conjugated oligothiophene. The presence of and/or the identity of and/or the quantity of one or more carbohydrates that is or are present on said object or in said sample is determined based on said detected detection signal from the LCO. The invention encompasses methods for carbohydrate detection by use of oligothiophene derivatives. The methods are quick, easy and direct and can be performed in real time as well as in situ.
1. A method for detection, identification and/or quantification of one or more carbohydrates, comprising the steps of:
a) contacting an object or a sample with a pentameric to 15-meric luminescent conjugated oligothiophene;
b) detecting at least one detection signal of the luminescent conjugated oligothiophene; and
c) based on said detected detection signal determining the presence, identity and/or quantity of the carbohydrate or carbohydrates on said object or in said sample.
2. The method according to claim 1 , wherein said luminescent conjugated oligothiophene is a pentameric or heptameric luminescent conjugated oligothiophene.
3. The method according to claim 1 , wherein said luminescent conjugated oligothiophene comprises one or more functional side chains.
4. The method according to claim 3 , wherein said functional side chain(s) is/are selected from amino acids, neurotransmitters, monosaccharides, polysaccharides, nucleic acids and combinations thereof.
5. The method according to claim 2 , wherein said heptameric luminescent conjugated oligothiophene is heptamer formyl thiophene acetic acid (h-FTAA) or heptamer hydrogen thiophene acetic acid (h-HTAA), and said pentameric luminescent conjugated oligothiophene is any of pentamer hydrogen thiophene acetic acid histidine (pHTA-His), pentamer hydrogen thiophene acetic acid lysine (pHTA-Lys), pentamer hydrogen thiophene ethanol amine (pHTEA), pentamer hydrogen thiophene imidazole (pHTIm), pentamer hydrogen thiophene acetic acid tyrosine (pHTA-Tyr), pentamer hydrogen thiophene acetic acid arginine (pHTA-Arg), pentamer hydrogen thiophene acetic acid aspartate (pHTA-Asp), and pentamer hydrogen thiophene acetic acid glutamate (pHTA-Glu).
6. The method according to claim 1 , wherein said detection signal is an optical signal.
7. The method according to claim 1 , wherein the luminescent conjugated oligothiophene is able to discriminate between at least two different carbohydrates.
8. The method according to claim 1 , wherein at least one of the carbohydrates is an insoluble carbohydrate.
9. The method according to claim 8 , wherein said insoluble carbohydrate is any of cellulose, chitin, β-glucan, alginate, amylose and glycogen, or combinations thereof.
10. The method according to claim 1 , wherein at least one of the carbohydrates is a soluble carbohydrate.
11. The method according to claim 10 , wherein said soluble carbohydrate is any of glucose, cellulobiose, heparin, chondroitin sulfate A, or combinations thereof.
12. The method according to claim 1 , wherein at least one of the carbohydrates is a structural carbohydrate, a storage carbohydrate, a glycoaminoglycan, an intermediate product of carbohydrate conversion and/or a metabolic substrate.
13. The method according to claim 1 , wherein at least step a) and/or step b) is or are carried out in vivo, in vitro or in situ.
14. The method according to claim 6 , wherein the optical signal is a fluorescence signal, a colorimetric signal, or an electrical signal.
15. The method according to claim 14 , wherein the electrical signal is conductivity.
16. The method according to claim 10 , wherein the sample is a tissue or blood sample.
17. The method according to claim 10 , wherein the sample is a water sample.
18. The method according to claim 8 , wherein the sample is from a carbohydrate manufacturing or carbohydrate extraction process.