Decellularized muscle matrix
Disclosed herein are muscle implants and methods of making muscle implants comprising one or more decellularized muscle matrices. The muscle matrices can, optionally, be joined to one or more decellularized dermal matrices. The muscle implants can be used to enhance muscle volume or to treat muscle damage, defects, and/or disorders. The decellularized muscle matrices in the implants retain at least some of the myofibers found in a muscle tissue prior to processing.
1. A method of preparing an implant, the method comprising
providing a muscle sample comprising natural myofibers; and
processing the muscle sample to produce a decellularized muscle matrix retaining at least some of the natural myofibers, the processing comprising:
contacting the muscle sample with a solution containing trypsin at a concentration of 0.1% to 0.5% w/v;
decellularizing the muscle sample to produce the decellularized muscle matrix; and
joining the decellularized muscle matrix to at least one decellularized dermal matrix to form a layered material comprising at least one decellularized muscle matrix layer and at least one decellularized dermal matrix layer in the form of a sheet.
2. The method of claim 1 , wherein the muscle sample is decellularized by contacting the sample with a decellularization solution comprising at least one of 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, sodium dodecyl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan monolaurate.
3. The method of claim 1 , wherein the decellularized muscle matrix retains about 20-80% of the natural myofibers present in the muscle sample.
4. The method of claim 1 , further comprising contacting the muscle sample with DNase.
5. The method of claim 1 , further comprising contacting the muscle sample with alpha-galactosidase.
6. The method of claim 1 , wherein the muscle sample is from an animal that lacks substantially all alpha-galactose moieties.
7. The method of claim 1 , wherein the at least one decellularized dermal matrix is at least two or at least three decellularized dermal matrices.
8. The method of claim 1 , wherein the decellularized muscle matrix and the at least one decellularized dermal matrix are joined using a fibrin glue or sutures.
9. The method of claim 1 , further comprising treating the decellularized muscle matrix to reduce bioburden.
10. The method of claim 9 , wherein the decellularized muscle matrix is exposed to e-beam radiation.
11. The method of claim 1 , further comprising packaging the layered material.
12. The method of claim 11 , further comprising sterilizing the layered material.