Differentiation marker and differentiation control of eye cell
The present invention relates to a differentiation marker and a differentiation controlling technique for an eye cell. More particularly, the present invention has attained an object of providing a differentiation marker for an eye cell among the aforementioned problems, by providing a marker for identifying a cell having a high proliferation ability among corneal endothelial cells and/or the differentiation ability of a corneal endothelial cell, the marker comprising GPR49/LGR5, as well as a detection agent or detection method for identifying a cell having a high proliferation ability among corneal endothelial cells and/or the differentiation ability of a corneal endothelial cell, comprising a substance binding to GPR49/LGR5. In addition, the present invention has attained an object of providing a differentiation controlling technique for an eye cell, by providing an agent for suppressing differentiation and/or promoting proliferation of an eye cell, comprising R-spondins.
1. A method for suppressing morphological change of a cultured corneal endothelial cell, comprising contacting one or more R-spondins with the corneal endothelial cell, wherein the R-spondins comprise R-spondin 1.
2. The method according to claim 1 , wherein the corneal endothelial cell is a cell which does not proliferate in the stationary state.
3. The method according to claim 1 , wherein the corneal endothelial cell comprises a corneal endothelial cell of a primate.
4. The method according to claim 1 , wherein the corneal endothelial cell comprises a human corneal endothelial cell.
5. The method according to claim 1 , wherein the corneal endothelial cell is in the confluent state.
6. The method according to claim 1 , wherein the corneal endothelial cell is provided in the form of a corneal tissue.
7. A method for suppressing morphological change and promoting proliferation of a cultured corneal endothelial cell, comprising contacting R-spondins with the corneal endothelial cell, wherein the R-spondins comprise R-spondin 1.
8. The method according to claim 7 , wherein the R-spondins further comprise an R-spondin selected from R-spondin-2, R-spondin 3 and R-spondin 4.
9. The method according to claim 7 , wherein the corneal endothelial cell is a cell which does not proliferate in the stationary state.
10. The method according to claim 7 , wherein the corneal endothelial cell comprises a corneal endothelial cell of a primate.
11. The method according to claim 7 , wherein the corneal endothelial cell comprises a human corneal endothelial cell.
12. The method according to claim 7 , wherein the corneal endothelial cell is in the confluent state.
13. The method according to claim 7 , wherein the corneal endothelial cell is provided in the form of a corneal tissue.