Aptamer-targetted antigen delivery
View Patent ↗A composition is provided comprising an oligonucleotide aptamer conjugated to an antigen, wherein the aptamer is directed against a cell-surface target of an antigen-presenting cell. Also provided are methods of delivering an antigen to a dendritic cell and of eliciting an immune response in a subject.
1. A composition comprising an oligonucleotide aptamer comprising the sequence set forth in SEQ ID NO:1 conjugated to an antigen.
2. The composition of claim 1 , wherein the aptamer comprises an oligoribonucleotide.
3. The composition of claim 1 , wherein the aptamer is PEGylated.
4. The composition of claim 1 , wherein the aptamer consists of the sequence set forth in SEQ ID NO:1.
5. The composition of claim 1 , wherein the aptamer further comprises a 3′ inverted dT.
6. The composition of claim 1 , further comprising one or more additional oligonucleotide aptamers conjugated to the antigen, wherein the aptamers are directed against a cell-surface target of an antigen-presenting cell.
7. The composition of claim 1 , wherein the antigen is a vaccine molecule.
8. The composition of claim 1 , wherein the composition further comprises an immunological adjuvant.
9. A method of presenting an antigen to a dendritic cell, comprising contacting the dendritic cell with the composition of claim 1 comprising the antigen.
10. A method of eliciting an immune response in a subject comprising administering to the subject the composition of claim 1 comprising the antigen, in an amount effective to elicit an immune response.
11. The method of claim 10 , wherein the antigen-presenting cell is a dendritic cell.
12. The method of claim 10 , wherein the antigen is a vaccine molecule.
13. The method of claim 10 , wherein the cell-surface target is a DEC-205 receptor molecule.