Methods and compositions for tissue adhesives
Disclosed herein are methods of connecting disrupted tissue, tissue repair, treating colorectal disorder and tissue welding. The methods comprise using a bioadhesive composition comprising ELP and light absorbing chromophores and irradiating the bioadhesive tissue.
1. A method of tissue repair comprising:
a) suturing a disrupted tissue with at least one suture comprising at least two fibers, wherein each fiber comprises a photothermally responsive composition comprising an ELP chemically conjugated to a light absorbing chromophore,
wherein the light absorbing chromophore is entrapped within the ELP; and
b) applying an effective amount of a directed light beam to the at least one suture and/or to the tissue to allow for the conversion of the light to heat.
2. The method of claim 1 , wherein the light absorbing chromophore comprises silver nanoparticles, gold nanorods, or gold nanoparticles, or mixtures thereof.
3. The method of claim 1 , wherein the light absorbing chromophore is gold nanorods.
4. The method of claim 1 , wherein the light absorbing chromophore crosslink the ELP.
5. The method of claim 1 , wherein the ELP comprises cysteine residues.
6. The method of claim 1 , wherein the ELP comprises at least twelve cysteine residues.
7. The method of claim 1 , wherein the directed light beam is a laser.
8. The method of claim 1 , wherein the light is near infrared.
9. The method of claim 1 , wherein the directed light beam generates a bulk temperature of at least a portion of the photothermally responsive bioadhesive composition above 60° C.
10. The method of claim 1 , wherein the directed light beam generates a bulk temperature of at least a portion of the photothermally responsive bioadhesive composition above 80° C.
11. The method of claim 1 , wherein the disrupted tissue is colorectal tissue, a blood or lymphatic vessel in the body, bowel, urinary tract tissue, or skin.
12. The method of claim 1 , wherein the bioadhesive composition further comprises an active agent.
13. The method of claim 12 , wherein the active agent comprises an antibacterial agent.
14. The method of claim 12 , wherein the active agent is a MMP inhibitor, a soluble factor, cytokine or growth factor.
15. The method of claim 14 , wherein a soluble factor comprises FGF (fibroblast growth factor), TGF-beta, EGF or other factors known as growth factors or cytokines, or known to be involved in wound healing and repair.
16. The method of claim 14 , wherein a MMP inhibitor is doxycycline.
17. The method of claim 1 , further comprising an encapsulated active agent.
18. The method of claim 1 , further comprising an active agent that is not encapsulated.
19. The method of claim 1 , wherein the photothermally responsive composition further comprises cells.
20. The method of claim 19 , wherein the cells comprise NCM460, fibroblasts or mixtures thereof.
21. The method of claim 19 , wherein the cells are encapsulated within the photothermally responsive bioadhesive composition.
22. The method of claim 1 , further comprising applying an effective amount of a photothermally responsive bioadhesive composition comprising an ELP chemically conjugated to a light absorbing chromophore to the sutured site and to the photothermally responsive bioadhesive composition, wherein the light absorbing chromophore is entrapped within the ELP.
23. The method of claim 1 , wherein the light absorbing chromophore in the fiber has a weight percentage between 0.5% and 8%.
24. The method of claim 1 , wherein the light absorbing chromophore in the fiber has a weight percentage of at least 4%.
25. The method of claim 1 , wherein the light absorbing chromophore in the fiber has a weight percentage between 4% and 6%.
26. The method of claim 1 , wherein the light absorbing chromophore in the fiber has a weight percentage of at least 5%.
27. The method of claim 1 , wherein the weight percentage of the light absorbing chromophore in the fiber is at least 8%.
28. A method of treating colorectal disease, comprising,
applying a photothermally responsive bioadhesive composition comprising an ELP chemically conjugated to a light absorbing chromophore to disrupted colorectal tissue, wherein the light absorbing chromophore is entrapped within the ELP; and
applying an effective amount of a directed light beam to the photothermally responsive bioadhesive composition and/or the tissue to allow for the conversion of the light to heat,
wherein disrupted colon tissue is repaired.